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yhaR
similar to enoyl CoA hydratase
Genomic Context
categories
[category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins][category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]Gene
Coordinates
1,061,491 1,062,258
The protein
Protein family
[SW|enoyl-CoA hydratase/isomerase family] (according to UniProt)Paralogous protein(s)
[protein|3111ECB66CD3CFF0494AA3DF4D646102CF3B0FA8|FadB]: [protein|D5CCFDE9312909B7E5B98876794569357791C76B|YngF]: Structure
[PDB|3P5M] (from ''Mycobacterium avium'', 33% identity) [pubmed|25613812][SW|Localization]
cell membrane (according to UniProt)Expression and Regulation
Operons
genes
[gene|25B45D6A90AD5152FDA71718B14767EA86ECA15B|yhaR]
description
[Pubmed|22383849]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]regulation
expression during spore [SW|germination] is strongly reduced under conditions of osmotic stress [Pubmed|27766092]view in new tabBiological materials
Mutant
MGNA-B498 (yhaR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1497 NBRP B. subtilis, Japan]BKE09880 ([gene|25B45D6A90AD5152FDA71718B14767EA86ECA15B|yhaR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAAAAGCACCTCCATGT, downstream forward: _UP4_TAACAGATCAGCCGGAAAGCBKK09880 ([gene|25B45D6A90AD5152FDA71718B14767EA86ECA15B|yhaR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAAAAGCACCTCCATGT, downstream forward: _UP4_TAACAGATCAGCCGGAAAGCReferences