SAVE THE DATE! 21st International Conference on Bacilli and Gram-Positive Bacteria. Prague, Czech Republic, June 7-11, 2021.

nasA

nitrate transporter

locus: BSU_03330

pI: 10.49

mw: 45.91 kDa

protein length: 401 aa

gene length: 1206 bp

function: nitrate uptake

product: nitrate transporter

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.8|Uptake of other small ions]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.1|Utilization of nitrate/ nitrite]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 362,937 364,142

The protein

Protein family

• [SW|major facilitator superfamily] (according to UniProt)
• Nitrate/nitrite porter (TC 2.A.1.8) family (with [protein|F14481DAA196868ACC1DE9EC8CBE593A2CBB6BE4|NarK], according to UniProt)

Paralogous protein(s)

• [protein|F14481DAA196868ACC1DE9EC8CBE593A2CBB6BE4|NarK]

Structure

• [PDB|4U4T] (NarK from E. coli, 24% identity) [pubmed|25959928]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|2DE7BEC614129384779F4C761E0792385DECC563|nasA]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: activation, [Pubmed|8799114], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]

  regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]) [Pubmed|8799114]

Biological materials

Mutant

• 1A972 ( ''nasA''::''phleo''), [Pubmed|7868621], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A972&Search=1A972 BGSC]
• BKE03330 ([gene|2DE7BEC614129384779F4C761E0792385DECC563|nasA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGATTCCCCTTTCTAG, downstream forward: _UP4_TAGGATTTTGACGGACACGC
• BKK03330 ([gene|2DE7BEC614129384779F4C761E0792385DECC563|nasA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGATTCCCCTTTCTAG, downstream forward: _UP4_TAGGATTTTGACGGACACGC

References

Reviews

• 22103536,11289299

Original publications

• 7836289,12823818,25755103,8799114,7868621,25959928