pucL

uricase, urate oxidase

locus: BSU_32450

pI: 5.48

mw: 56.39 kDa

protein length: 494 aa

gene length: 1485 bp

function: purine utilization

product: uricase

essential: no

ec: 1.7.3.3

synonyms: yunL

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.1|Utilization of nucleotides]

Gene

Coordinates: 3,333,162 3,334,646

The protein

Catalyzed reaction/ biological activity

• 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate + H⁺ --> (S)-allantoin + CO₂ (according to UniProt)
• H₂O + O₂ + urate --> 5-hydroxyisourate + H₂O₂ (according to UniProt)

Protein family

• N-terminal part: OHCU decarboxylase family (single member, according to UniProt)
• C-terminal part: uricase family (single member, according to UniProt)

Structure

• [PDB|6A4M]

Expression and Regulation

Operons

• genes: [gene|53950BFDC128DE46F3F43BDB9D0FDB3D645152DF|pucJ]-[gene|3162EF36F4441A1E4EBBFDAD19F6768D8EF21B29|pucK]-[gene|2E7DEC53A130247E66225E87950D702E462C324F|pucL]-[gene|A2F93FFE225DFDBCBB3EA079BEF84C79465F0E5C|pucM]
  description: [Pubmed|12029039]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12029039], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: activation, [Pubmed|12823818], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]
  • [protein|52C1601482C26400A524E880334BB801F832D6ED|PucR]: activation, [Pubmed|12029039], in [regulon|52C1601482C26400A524E880334BB801F832D6ED|PucR regulon]

  regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|12823818]

• genes: [gene|52C1601482C26400A524E880334BB801F832D6ED|pucR]-[gene|53950BFDC128DE46F3F43BDB9D0FDB3D645152DF|pucJ]-[gene|3162EF36F4441A1E4EBBFDAD19F6768D8EF21B29|pucK]-[gene|2E7DEC53A130247E66225E87950D702E462C324F|pucL]-[gene|A2F93FFE225DFDBCBB3EA079BEF84C79465F0E5C|pucM]
  description: [Pubmed|12823818]

  regulatory mechanism

  • [protein|52C1601482C26400A524E880334BB801F832D6ED|PucR]: auto-repression, [Pubmed|12029039], in [regulon|52C1601482C26400A524E880334BB801F832D6ED|PucR regulon]
  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: activation, [Pubmed|25755103], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]

  regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|12823818]

Biological materials

Mutant

• MGNA-A937 (yunL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/937 NBRP B. subtilis, Japan]
• BKE32450 ([gene|2E7DEC53A130247E66225E87950D702E462C324F|pucL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTGAACATTGGCATCT, downstream forward: _UP4_AAATGTCGGAGCCTGAAAGC
• BKK32450 ([gene|2E7DEC53A130247E66225E87950D702E462C324F|pucL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTGAACATTGGCATCT, downstream forward: _UP4_AAATGTCGGAGCCTGAAAGC

References

• 11344136,25755103,12823818,12029039,20168977,28531234