nprE [Wed Jun 15 2016 16:37:37 GMT+0200 (CEST)]

extracellular neutral protease B

locus: BSU14700

pI: 7.00

mw: 56.00 kDa

function: degradation of proteins

product: extracellular neutral protease B

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.5|Utilization of proteins]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.2|Extracellular feeding proteases]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

[SW|Categories] containing this gene/protein

• [SW|utilization of nitrogen sources other than amino acids], [SW|proteolysis]

This gene is a member of the following [SW|regulons]

• [SW|AbrB regulon], [SW|CodY regulon], [SW|ScoC regulon]

Gene

Coordinates on the chromosome (coding sequence): 1,540,036 -> 1,541,601

The protein

Catalyzed reaction/ biological activity

• Similar, but not identical, to that of thermolysin (according to Swiss-Prot)

Protein family

• peptidase M4 family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|search|NprB]

Structure

• [PDB|1NPC] (of ''Bacillus cereus'')

[SW|Localization]

• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operon

• ''nprE'' (according to [http://dbtbs.hgc.jp/COG/prom/nprE.html DBTBS])

Regulation

• repressed during exponential growth ([protein|search|ScoC]) [Pubmed|1906467]

Regulatory mechanism

• [protein|search|ScoC]: transcription repression [Pubmed|1906467]
• [protein|search|AbrB]: transcription repression [Pubmed|20817675]
• [SW|CodY]: transcription repression [Pubmed|26728191]

Additional information

• in a triple ''[SW|abrB] [SW|codY] [SW|scoC]'' mutant, expression occurs during exponential growth [Pubmed|26728191]

Biological materials

Mutant

• BKE14700 ( ''ΔnprE::erm trpC2''), available at BGSC
• KO7 (''ΔnprE ΔaprE Δepr Δmpr ΔnprB Δvpr Δbpr''), available as BGSC 1A1133

References

Reviews

• 20735481

Original publications

• 1906467,18957862,20817675,24115457,26728191