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21st International Conference on Bacilli and Gram-Positive Bacteria. Prague, Czech Republic, June 7-11, 2021.
pyridoxal phosphate-binding protein, involved in controlling the availability of coenzyme A
control of the CoA pool
pyridoxal phosphate-binding protein
categories[category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]
Catalyzed reaction/ biological activitywhile the protein was described as an amino acid racemase important for biofilm formation [Pubmed|20431016], no racemase activity was detected in ''in vitro'' assays with a functionally active protein [Pubmed|24097949]
Protein familypyridoxal phosphate-binding protein YggS/PROSC family (single member, according to UniProt)
[SW|Cofactors]pyridoxal phosphate [Pubmed|24097949]
Structure[PDB|1W8G] (the structure of YggS from ''E. coli'' 33% identity, 61% similarity)
Expression and Regulation
regulatory mechanism[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
regulationrepressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]view in new tab
MutantMGNA-B363 (ylmE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1362 NBRP B. subtilis, Japan]BKE15380 ([gene|31789A170CB624BF8210C915F40007F802F5C81B|ylmE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATTATCAACAACACGCAAGA, downstream forward: _UP4_AATGAAACAGGGGGTGTACABKK15380 ([gene|31789A170CB624BF8210C915F40007F802F5C81B|ylmE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATTATCAACAACACGCAAGA, downstream forward: _UP4_AATGAAACAGGGGGTGTACA