SubtiBank SubtiBank
glyS
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

glyS

glycyl-tRNA synthetase (beta subunit)
locus
BSU25260
pI
4.97
mw
76.05 kDa
protein length
679 aa Sequence Blast
gene length
2037 bp Sequence Blast
function
translation
product
glycyl-tRNA synthetase (beta subunit)
essential
yes
ec
6.1.1.14
synonyms
yqfK

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    2,605,730 → 2,607,769

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly) (according to Swiss-Prot)
  • Protein family

  • class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Structure

  • [PDB|1J5W] (from ''Thermotoga maritima'', 55% identity, 72% similarity)
  • Expression and Regulation

    Operons

    genes
    [gene|FD84CF3E05B81BD38D8C8FF5B04728FB3912D9B5|glyQ]-[gene|337BF226112A48C39A24E6D5C7A63F864502914C|glyS]
    description
    (according to [http://dbtbs.hgc.jp/COG/prom/glyQS.html DBTBS]) null

    regulatory mechanism

  • [regulon|T-box|T-box]: anti-termination, [Pubmed|19258532,28531275], in [regulon|T-box|T-box]
  • regulation

  • induced by glycine limitation ([protein|search|T-box]) [Pubmed|19258532,28531275]
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    References

  • 15890195,15663928,19258532,15292140,16741230,26229106