SAVE THE DATE! 21st International Conference on Bacilli and Gram-Positive Bacteria. Prague, Czech Republic, June 7-11, 2021.

mgsA [2019-02-19 17:28:49]

methylglyoxal synthase

locus: BSU22480

pI: 4.92

mw: 14.99 kDa

protein length: 137 aa

gene length: 414 bp

function: bypass of glycolysis

product: methylglyoxal synthase

essential: no

ec: 4.2.3.3

synonyms: ypjF,jojF

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.1|Glycolysis]

Gene

Coordinates: 2,358,911 2,359,324

The protein

Catalyzed reaction/ biological activity

• Glycerone phosphate = methylglyoxal + phosphate (according to Swiss-Prot)

Effectors of protein activity

• non-phosphorylated [protein|A269774F2FDC94F93BA5F1360FFFE754B50383AD|Crh] interacts with [protein|341F198B2BD4261B20C00181D084A7C5E33E39B3|MgsA] to inhibit its activity [Pubmed|29514981,21992469]

Structure

• [PDB|6F2C] [pubmed|29514981]

Expression and Regulation

Operons

• genes: [gene|A828298EA2A002538876FBA6113648AAD9C4C317|ypjD]-[gene|5AB90032031653A913E15B26B3E1AE246E95AABF|dapB]-[gene|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA]-[gene|60367F0396612C8A6491FD92A73B30BED7215506|bshB1]-[gene|187C78441816FC74B0FCCE34997E8AEC0A80B114|bshA]-[gene|4C4FA59FF5D100E941EF470C0C54E85681ADC74F|cca]-[gene|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA]
  description: [Pubmed|20308541]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|23894131], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|23894131], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

  regulation

  • induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]

Biological materials

Mutant

• MGNA-A440 ([gene|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA]::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/440 NBRP B. subtilis, Japan]
• GP67 ([gene|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA]::''tet'') [Pubmed|21992469], available in [SW|Jrg Stlke]'s lab
• GP84 (''mgsA''::''pX2''(''cat'')), available in [SW|Jrg Stlke]'s lab
• BKE22480 ([gene|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATTTTCATTGTTTATCCCC, downstream forward: _UP4_GACCTTCTTCGGGGAGAAGA
• BKK22480 ([gene|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATTTTCATTGTTTATCCCC, downstream forward: _UP4_GACCTTCTTCGGGGAGAAGA

Expression vectors

• pGP1301 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jrg Stlke]'s lab
• pGP1180 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Jrg Stlke]'s lab
• pGP1181 (C-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP382]), available in [SW|Jrg Stlke]'s lab
• pGP2207 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP1459]), available in [SW|Jrg Stlke]'s lab
• pGP2505 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jrg Stlke]'s lab

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jrg Stlke]'s lab [Pubmed|21992469]

FLAG-tag construct

• GP86 (spc, based on [SW|pGP1331]) [Pubmed|21992469], available in [SW|Jrg Stlke]'s lab

labs

• [SW|Boris Grke], University of Vienna, Austria
• [SW|Jrg Stlke], University of Gttingen, Germany, [http://genmibio.uni-goettingen.de/index.php?id=90 Homepage]

References

• 10368300,21992469,20308541,22074179,23894131,28807600,29514981