SubtiBank SubtiBank
mgsA [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]
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mgsA [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]

methylglyoxal synthase
locus
BSU22480
pI
4.92
mw
14.00 kDa
function
bypass of glycolysis
product
methylglyoxal synthase
essential
no
ec
4.2.3.3
synonyms
ypjF, jojF

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.1|Glycolysis]
  • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism]
  • This gene is a member of the following [SW|regulons]

  • [SW|Spx regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    2,358,911 -> 2,359,324

    The protein

    Catalyzed reaction/ biological activity

  • Glycerone phosphate = methylglyoxal + phosphate (according to Swiss-Prot)
  • Protein family

  • RNase Z family (according to Swiss-Prot)
  • Effectors of protein activity

  • non-phosphorylated [protein|search|Crh] interacts with [protein|search|MgsA] to inhibit its activity [Pubmed|21992469]
  • Structure

  • [PDB|1B93] (from ''Escherichia coli'', 50% identity, 73% similarity) [Pubmed|10368300]
  • [SW|Interactions]

  • [protein|search|Crh]-[protein|search|MgsA] (only non-phosphorylated [protein|search|Crh] interacts with [protein|search|MgsA]) [Pubmed|21992469]
  • Expression and Regulation

    Operon

  • ''[protein|search|ypjD]-[protein|search|dapB]-[protein|search|mgsA]-[SW|bshB1]-[protein|search|bshA]-[protein|search|cca]-[protein|search|birA]'' [Pubmed|20308541]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|23894131]
  • Regulation

  • induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]
  • Regulatory mechanism

  • [protein|search|Spx]: transcription activation [Pubmed|23894131]
  • Biological materials

    Mutant

  • GP67 (''mgsA''::''tet'') [Pubmed|21992469], available in [SW|Jörg Stülke]'s lab
  • GP84 (''mgsA''::''pX2''(''cat'')), available in [SW|Jörg Stülke]'s lab
  • Expression vector

  • pGP1301 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
  • pGP1180 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab
  • pGP1181 (C-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab
  • pGP2207 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP1459]), available in [SW|Jörg Stülke]'s lab
  • pGP2505 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab [Pubmed|21992469]
  • FLAG-tag construct

  • GP86 (spc, based on [SW|pGP1331]) [Pubmed|21992469], available in [SW|Jörg Stülke]'s lab
  • Labs working on this gene/protein

  • [SW|Boris Görke], University of Vienna, Austria
  • [SW|Jörg Stülke], University of Göttingen, Germany, [http://genmibio.uni-goettingen.de/index.php?id=90 Homepage]
  • References

  • 10368300,21992469,20308541,22074179,23894131