dat

locus: BSU_09670

pI: 4.87

mw: 31.03 kDa

protein length: 282 aa

gene length: 849 bp

function: peptidoglycan precursor biosynthesis

product: D-alanine aminotransferase

essential: no

ec: 2.6.1.21

synonyms: yheM

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.15|Biosynthesis/acquisition of L- and D-alanine]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]

Gene

Coordinates: 1,041,994 1,042,842

The protein

Catalyzed reaction/ biological activity

• 2-oxoglutarate + D-alanine --> D-glutamate + pyruvate (according to UniProt)

Protein family

• [SW|Class-IV pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

[SW|Cofactors]

• PLP

Structure

• [PDB|1G2W] (E177S Mutant, Geobacillus stearothermophilus, 43% identity)

Expression and Regulation

Operons

• genes: [gene|38989B6CB025AE110709E2EA2025EFD425328FFA|dat]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B489 (yheM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1488 NBRP B. subtilis, Japan]
• BKE09670 ([gene|38989B6CB025AE110709E2EA2025EFD425328FFA|dat]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAGCCCTCCCTTTTC, downstream forward: _UP4_TAAATGAACGAAGAAAAGAG
• BKK09670 ([gene|38989B6CB025AE110709E2EA2025EFD425328FFA|dat]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAGCCCTCCCTTTTC, downstream forward: _UP4_TAAATGAACGAAGAAAAGAG

References

• 9003455,17183219