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pspA [2018-06-29 14:45:53]
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pspA [2018-06-29 14:45:53]

phage shock protein A homolog
locus
BSU06180
pI
5.78
mw
24.99 kDa
protein length
227 aa Sequence Blast
gene length
681 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
ydjF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]

    • Gene

    Coordinates
    671,245 → 671,928

    Phenotypes of a mutant

  • more sensitive to nisin [Pubmed|23980836]

    • The protein

    Protein family

  • pspA/IM30 family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|AE4BF12C368468C553EED7A696D5EFC63F56CA39|LiaH]

    • Structure

  • [PDB|4WHE] (from E. coli, 34% identity, corresponds to aa 1 ... 133)

    • [SW|Localization]

  • cytoplasma (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|3B6F4FBB19401069E827EAF5835DD15AAA3C3487|pspA]-[gene|F93381205C61665820D034A384BD60410AAE84C1|ydjG]-[gene|938B55332DFB2B465D0D5AD530550CED5AC30A92|ydjH]-[gene|CA4EF8385EAA4BBC47FF0D998C820D9F36F5F94D|ydjI]
    description
    [Pubmed|11454200]

    sigma factors

  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|11454200], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]

    • regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|18840696,20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

    • regulation

  • induced by alkaline shock ([protein|search|SigW]) [Pubmed|11454200]
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    Biological materials

    Mutant

  • MGNA-C430 (yqgI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2428 NBRP B. subtilis, Japan]
  • BKE06180 (Δ[gene|3B6F4FBB19401069E827EAF5835DD15AAA3C3487|pspA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATATCGTTCCTCCTAA, downstream forward: _UP4_TAATACATAGGAGGCCGCAG
  • BKK06180 (Δ[gene|3B6F4FBB19401069E827EAF5835DD15AAA3C3487|pspA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATATCGTTCCTCCTAA, downstream forward: _UP4_TAATACATAGGAGGCCGCAG

    • References

  • 11454200,18840696,22926563,23980836,27791134