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yhcW

putative glycerol-3-phosphatase

locus: BSU_09240

pI: 4.63

mw: 24.53 kDa

protein length: 220 aa

gene length: 663 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 997,724 998,386

The protein

Catalyzed reaction/ biological activity

• glycerol-3-phosphate - glycerol + inorganic phosphate [Pubmed|22353596]

Protein family

• [SW|HAD superfamily] (according to UniProt)
• CbbY/CbbZ/Gph/YieH family (with [protein|CAFAE305B1771DD89E81F75A6713161393BE99A0|PgcM], according to UniProt)

Paralogous protein(s)

• [protein|CAFAE305B1771DD89E81F75A6713161393BE99A0|PgcM]:

Structure

• [PDB|4UAR] (from ''Rhodobacter Sphaeroides'', 32% identity)

Expression and Regulation

Operons

• genes: [gene|3DB9048B54C47E262997A2A5A0556AE607F93EA9|yhcW]-[gene|BDAA216E832B8EB267CED6456A0045254DD0E97D|yhcX]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A660 (yhcW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/660 NBRP B. subtilis, Japan]
• BKE09240 ([gene|3DB9048B54C47E262997A2A5A0556AE607F93EA9|yhcW]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTGGGTACAACTCCTTTA, downstream forward: _UP4_CAAAACTAGAAAGGAGCGAG
• BKK09240 ([gene|3DB9048B54C47E262997A2A5A0556AE607F93EA9|yhcW]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTGGGTACAACTCCTTTA, downstream forward: _UP4_CAAAACTAGAAAGGAGCGAG

References

• 22353596,22383849