SubtiBank SubtiBank
queG
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queG

epoxyqueuosine reductase
locus
BSU08910
pI
7.35
mw
48.37 kDa
protein length
435 aa Sequence Blast
gene length
1305 bp Sequence Blast
function
synthesis of queuosine, tRNA modification
product
epoxyqueuosine reductase
essential
no
synonyms
ygaP,yhbA

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
  • Gene

    Coordinates
    967,935 → 969,095

    The protein

    Catalyzed reaction/ biological activity

  • conversion of epoxyqueuosine to queuosine [Pubmed|21502530]
  • [SW|Cofactors]

  • cobalamin
  • Fe-S cluster [Pubmed|21502530]
  • Structure

  • [PDB|5D08] [Pubmed|27638883]
  • Expression and Regulation

    Operons

    genes
    [gene|3F22BD8DB7BEC2701B960546AC683603DFCADCCE|queG]
    description
    [pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-A241 (yhbA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/241 NBRP B. subtilis, Japan]
  • BKE08910 (Δ[gene|3F22BD8DB7BEC2701B960546AC683603DFCADCCE|queG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTGCACAGCCTCCT, downstream forward: _UP4_TGACATACGTATAGAGCAGA
  • BKK08910 (Δ[gene|3F22BD8DB7BEC2701B960546AC683603DFCADCCE|queG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTGCACAGCCTCCT, downstream forward: _UP4_TGACATACGTATAGAGCAGA
  • References

  • 26230193,21502530,27638883