SubtiBank SubtiBank
zwf [2018-04-11 10:13:03]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

zwf [2018-04-11 10:13:03]

glucose 6-phosphate dehydrogenase, pentose-phosphate pathway
locus
BSU23850
pI
5.28
mw
55.49 kDa
protein length
489 aa Sequence Blast
gene length
1467 bp Sequence Blast
function
initiation of the pentose phosphate pathway
product
glucose 6-phosphate dehydrogenase
essential
no
ec
1.1.1.49
synonyms
yqjJ

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.3|Pentose phosphate pathway]
  • Gene

    Coordinates
    2,479,156 → 2,480,625

    The protein

    Catalyzed reaction/ biological activity

  • D-glucose 6-phosphate + NADP+ = D-glucono-1,5-lactone 6-phosphate + NADPH (according to Swiss-Prot)
  • Protein family

  • glucose-6-phosphate dehydrogenase family (according to Swiss-Prot)
  • [SW|Cofactors]

  • Mg2+, Mn2+, Ca2+
  • Effectors of protein activity

  • NAD+, NADP+ and NADPH affect the enzyme kinetic [Pubmed|6292660]
  • Structure

  • [PDB|1DPG] (from ''Leuconostoc Mesenteroides'', 45% identity, 62% similarity) [Pubmed|7881907]
  • Additional information

  • The enzyme is a dimer [Pubmed|6292660]
  • Expression and Regulation

    Operons

    genes
    [gene|43CD2581DA421CABC66E30389640CC86E6B2D800|zwf]
    description
    [Pubmed|12850135]

    regulation

  • constitutive [Pubmed|12850135]
  • view in new tab

    Biological materials

    Mutant

  • MGNA-C392 (yqjJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2390 NBRP B. subtilis, Japan]
  • SM-NB3 (''zwf-spc''), available in [SW|Anne Galinier]'s and [SW|Boris Görke]'s labs
  • BKE23850 (Δ[gene|43CD2581DA421CABC66E30389640CC86E6B2D800|zwf]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTAAAAGTACCTCACTTT, downstream forward: _UP4_TAATAAGAAGAAAAAAGCCG
  • BKK23850 (Δ[gene|43CD2581DA421CABC66E30389640CC86E6B2D800|zwf]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTAAAAGTACCTCACTTT, downstream forward: _UP4_TAATAAGAAGAAAAAAGCCG
  • FLAG-tag construct

  • GP1401 (spc, based on [SW|pGP1331]), available in the [SW|Stülke] lab
  • Antibody

  • **
  • References

  • 6292660,12850135,19779711,7881907,24571712