SubtiBank SubtiBank
ispG
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ispG

similar to peptidoglycan acetylation, 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase, 6th step in the MEP pathway of isoprenoid biosynthesis
locus
BSU_25070
pI
6.80
mw
40.42 kDa
protein length
377 aa Sequence Blast
gene length
1134 bp Sequence Blast
function
MEP pathway of isoprenoid biosynthesis
product
1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase
essential
yes
ec
1.17.7.3
synonyms
yqfY

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.3|Biosynthesis of isoprenoids]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    2,589,123 2,590,256

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H+ + H2O + oxidized [flavodoxin] --> 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + reduced [flavodoxin] (according to UniProt)
  • Protein family

  • ispG family (single member, according to UniProt)
  • [SW|Cofactors]

  • Fe-S cluster [pubmed|29292548]
  • Structure

  • [PDB|3NOY] (from ''Aquifex aeolicus'', 51% identity) [Pubmed|20932974]
  • [SW|Localization]

  • secreted (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|144A8B3F5E534F3FD6EFC1E9A5ED650E09DCE885|yqfZ]-[gene|4992F5C2ED7DE37C08D5715F7E8851F5FDB51059|ispG]
    description
    [Pubmed|12662922]

    sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|12662922], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
  • regulatory mechanism

  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID]: repression, [Pubmed|15383836], in [regulon|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID regulon]
  • regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE], [SW|SpoIIID]) [Pubmed|12662922,15383836]
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    References

  • 12662922,15383836,17458547,23832336,23840410,20932974