SAVE THE DATE!
21st International Conference on Bacilli and Gram-Positive Bacteria. Prague, Czech Republic, June 7-11, 2021.
cwlJ
spore coat protein, cell wall hydrolase
function
spore germination
product
spore coat protein, cell wall hydrolase
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.5|Cell wall/ other][category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.1|Spore coat proteins] → [category|SW 4.2.1.1.3|Class III][category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.4|Germination] → [category|SW 4.2.4.2|Additional germination proteins]Gene
Coordinates
282,469 282,897
The protein
Protein family
cwlJ family (single member, according to UniProt)Effectors of protein activity
activity depends on [protein|77C3C4ADCBD38EEA99C44850CAEB310C59B6C938|SwsB] [pubmed|31871031][SW|Localization]
outer edge of the spore cortex [Pubmed|12177332]inner spore coat, localization depends on [protein|CEBEC9CECCF445C40D793E61A2CD23175631A473|SafA], [protein|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|CotE], and [protein|1CA990DA55B6F879968C431A703E0478F5564A1A|GerQ] [Pubmed|31871031,22171814]Expression and Regulation
Operons
genes
[gene|49ED84B48CEC09493B6056D03D2A57578770CE15|cwlJ]
description
[Pubmed|9515903]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|9515903,15699190], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon][protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15383836], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]regulatory mechanism
[protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID]: activation, [Pubmed|15383836], in [regulon|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID regulon]regulation
expressed during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE], [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK], [SW|SpoIIID]) [Pubmed|15383836,9515903,15699190]strongly repressed in the presence of salt (1.2 M NaCl) [pubmed|32419322]view in new tabBiological materials
Mutant
1G19 ( ''cwlJ''::''tet''), [Pubmed|11466292], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1G19&Search=1G19 BGSC]BKE02600 ([gene|49ED84B48CEC09493B6056D03D2A57578770CE15|cwlJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACGTCACCTCCCCTTG, downstream forward: _UP4_TAGAGACAGGACACCGTTCABKK02600 ([gene|49ED84B48CEC09493B6056D03D2A57578770CE15|cwlJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACGTCACCTCCCCTTG, downstream forward: _UP4_TAGAGACAGGACACCGTTCAReferences
Reviews
Original publications
16936016,11807087,9515903,15699190,15317760,11466292,20435722,12177332,23543708,23746146,15383836,22171814,26187959,31871031,32419322