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iolT

major transporter for inositol

locus: BSU_06230

pI: 9.57

mw: 51.47 kDa

protein length: 473 aa

gene length: 1422 bp

function: myo-inositol uptake

product: major transporter for inositol

essential: no

synonyms: ydjK

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.3|Carbohydrate transporter]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.16|Utilization of inositol]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 676,442 677,863

The protein

Catalyzed reaction/ biological activity

• transport of myo-inositol [Pubmed|20530884]

Protein family

• [SW|major facilitator superfamily] (according to UniProt)
• [SW|Sugar transporter (TC 2.A.1.1) family] (according to UniProt)

Paralogous protein(s)

• [protein|4A77316AA94F9C11DB70AB76711AACBD28098AA1|IolT], [protein|770D9A5BEEEABE9C3FC772E74001329206ECFAB3|AraE], [protein|7CE5A42042E5D52768735E795DB805530691D8A6|YwtG], [protein|8B0701B5694ABA8142476CB896E590FE1981D7DB|YfiG], [protein|A84AC4E40E7C722E27AE9C24ACECFEABD51B57BE|YncC], [protein|0757FAEB38AA1C599C5067794AF83A4BA5912DC9|CsbC], [protein|4A77316AA94F9C11DB70AB76711AACBD28098AA1|IolT]

Structure

• [PDB|4LDS] (glucose transporter from Staphylococcus epidermidis, 36% identity) [pubmed|24127585]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|4A77316AA94F9C11DB70AB76711AACBD28098AA1|iolT]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|5F4ABBC8ECAD6F6C10EC39B2B0F0BC73F11522CE|IolR]: repression, [pubmed|11807058], in [regulon|5F4ABBC8ECAD6F6C10EC39B2B0F0BC73F11522CE|IolR regulon]

  regulation

  • induced in the presence of inositol ([protein|5F4ABBC8ECAD6F6C10EC39B2B0F0BC73F11522CE|IolR]) [Pubmed|11807058]

Biological materials

Mutant

• MGNA-C218 (ydjK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2216 NBRP B. subtilis, Japan]
• BKE06230 ([gene|4A77316AA94F9C11DB70AB76711AACBD28098AA1|iolT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTGTTTCCCCCAGTCT, downstream forward: _UP4_TAATTTAAAAAAGAATCCGC
• BKK06230 ([gene|4A77316AA94F9C11DB70AB76711AACBD28098AA1|iolT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTGTTTCCCCCAGTCT, downstream forward: _UP4_TAATTTAAAAAAGAATCCGC

References

• 11807058,20530884,28431560,24127585