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ydiG
molybdenum cofactor biosynthesis protein
product
molybdenum cofactor biosynthesis protein
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.10|Biosynthesis of molybdopterin]Gene
Coordinates
646,582 647,094
The protein
Catalyzed reaction/ biological activity
involved in the first step of molybdenum cofactor biosynthesis (together with [protein|19EB8E7CBC0E8D7A6429ADFFF93D1F71EFE3FF11|MoaA])(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate --> cyclic pyranopterin phosphate + diphosphate (according to UniProt)Protein family
moaC family (single member, according to UniProt)Structure
[PDB|1EKR] (the protein from ''E. coli'', 51% identity, 78% similarity) [Pubmed|10903949]Expression and Regulation
Operons
genes
[gene|4A8D74B83E20FA138ED66F0D8FD456B69B740D92|ydiG]-[gene|B5EF521437323EF43F08E5EFDB5C798616CA499A|rex]-[gene|780FDF3DD260287428B397FFE7F44DDF1917381C|tatAY]-[gene|E60FC9C9B273DD6B07B7B95819525DE5F35113CE|tatCY]
description
[Pubmed|22383849]
regulation
constitutitvely expressed [Pubmed|22383849]the mRNA is processed between [gene|B5EF521437323EF43F08E5EFDB5C798616CA499A|rex] and [gene|780FDF3DD260287428B397FFE7F44DDF1917381C|tatAY] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]view in new tabBiological materials
Mutant
MGNA-C200 (ydiG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2198 NBRP B. subtilis, Japan]BKE05960 ([gene|4A8D74B83E20FA138ED66F0D8FD456B69B740D92|ydiG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTAAAACGCTCCTTAA, downstream forward: _UP4_GAGTATAATTTGGAGGACCABKK05960 ([gene|4A8D74B83E20FA138ED66F0D8FD456B69B740D92|ydiG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTAAAACGCTCCTTAA, downstream forward: _UP4_GAGTATAATTTGGAGGACCAReferences
Reviews
Original publications