bkdR

transcriptional activator of the ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB operon

locus: BSU24100

pI: 6.24

mw: 77.75 kDa

protein length: 692 aa

gene length: 2076 bp

function: regulation of branched-chain amino acid utilization,

product: transcriptional activator (for SigL-dependent promoter))

essential: no

synonyms: yqiR

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.9|Utilization of branched-chain amino acids]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 2,504,789 → 2,506,867

Phenotypes of a mutant

• The mutant is cold-sensitive [http://www.ncbi.nlm.nih.gov/sites/entrez/16585774 PubMed]

The protein

Protein family

• [SW|Transcription factors activating transcription at SigL-dependent promoters]

[SW|Domains]

• [SW|PAS domain]

Expression and Regulation

Operons

• genes: [gene|4CEBD81F485DD0B660E297FFC34A0F5270652184|bkdR]
  description: [Pubmed|12823818]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|10094682], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

  regulation

  • repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|10094682]

Biological materials

Mutant

• MGNA-C375 (yqiR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2373 NBRP B. subtilis, Japan]
• 1A913 ( ''bkdR''::''kan''), [Pubmed|16585774], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A913&Search=1A913 BGSC] and in [SW|Jörg Stülke]'s lab

References

• 16585774,10094682,23123912,12823818,21906631