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yhdN
general stress protein, broad specificity aldo-keto reductase that converts MG to acetol
function
detoxification of methylglyoxal
product
aldo/keto reductase, specific for NADP
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]Gene
Coordinates
1,030,265 1,031,260
Phenotypes of a mutant
increased sensitivity to methylglyoxal [Pubmed|24330391]The protein
Catalyzed reaction/ biological activity
methylglyoxal ---> acetol [Pubmed|24330391]Protein family
[SW|Aldo/keto reductase family] (according to UniProt)Paralogous protein(s)
[protein|B73D729097C00BE6F3C7FF1708738783EE93C6FB|YccK], [protein|D0A2506FDCEA41D2A8910AC2837B46E0B311D63B|YcsN], [protein|F1AFFACD36C75702B0BB8F0B4241A9688FB6253C|IolS] [SW|Cofactors]
NADPH [Pubmed|24330391]Structure
[PDB|1PZ1] [Pubmed|15019785]Expression and Regulation
Operons
genes
[gene|4DF9B105ABA64AFD5106EE75FCC9EFAB52BEFC83|yhdN]-[gene|FC05D5FC95CEDC4B4A04040C86170D185528B9FF|plsC]
description
[Pubmed|10220166]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|10220166,15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]regulatory mechanism
[protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR]: repression, [Pubmed|12737802], in [regulon|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR regulon]regulation
induced by stress ([protein|search|SigB]) [Pubmed|10220166,15805528]view in new tabBiological materials
Mutant
MGNA-A695 (yhdN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/695 NBRP B. subtilis, Japan]BKE09530 ([gene|4DF9B105ABA64AFD5106EE75FCC9EFAB52BEFC83|yhdN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTGCCACTCCTTCACT, downstream forward: _UP4_TAACAAAAGCTGAGGGCGAABKK09530 ([gene|4DF9B105ABA64AFD5106EE75FCC9EFAB52BEFC83|yhdN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTGCCACTCCTTCACT, downstream forward: _UP4_TAACAAAAGCTGAGGGCGAAReferences
10220166,15019785,24330391,12737802,15805528