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ribE
riboflavin synthase (alpha subunit)
function
riboflavin biosynthesis
product
riboflavin synthase (alpha subunit)
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.2|Biosynthesis/ acquisition of riboflavin/ FAD]Gene
Coordinates
2,429,600 2,430,247
The protein
Catalyzed reaction/ biological activity
2 6,7-dimethyl-8-(1-D-ribityl)lumazine + H+ --> 5-amino-6-(D-ribitylamino)uracil + riboflavin (according to UniProt)Structure
[PDB|1I8D] (from ''E. coli'', 36% identity, 59% similarity) [Pubmed|11377200], , [PDB|1RVV] (the [protein|4E18BD4B084094EE4FBC8FE2AC95B14581D20C0F|RibE])3-([protein|2A5DCBFAB51996F0DDEC964D5D178274AF86CE69|RibH])60 lumazine synthase/riboflavin synthase complex) [Pubmed|7473709]Expression and Regulation
Operons
genes
[gene|3DFCF7C5FE15C9A3E9E7E4B0DA8E926B85DBD180|ribD]-[gene|4E18BD4B084094EE4FBC8FE2AC95B14581D20C0F|ribE]-[gene|F07B7062C850106A95C15978E36DA500F8B089D6|ribA]-[gene|2A5DCBFAB51996F0DDEC964D5D178274AF86CE69|ribH]-[gene|8146A052E4E7844F781E7169C264EC927DEB7387|ribT]
description
[Pubmed|8159171]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8159171], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[regulon|FMN-box|FMN-box]: transcription termination, via [SW|FMN-box] in the presence of FMN or FMNH2, this is counter-acted upon binding of [protein|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|RibR], in [regulon|FMN-box|FMN-box]regulation
expressed in the absence of FMN ([SW|FMN-box]) [Pubmed|15808508]binding of FMN to the [SW|FMN-box] RNA is facilitated by [protein|43CFA3477B4EEB4CE0B2DF63ED2B5EDB171E79C7|NusG]-mediated transcription pausing [pubmed|32817529]the [SW|FMN-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]view in new tabBiological materials
Mutant
GP203 (erm), available in [SW|Jörg Stülke]'s labBKE23270 ([gene|4E18BD4B084094EE4FBC8FE2AC95B14581D20C0F|ribE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATTCCTGTAAACATGGTCA, downstream forward: _UP4_GGCTTTTAGAGAGGAAGATTBKK23270 ([gene|4E18BD4B084094EE4FBC8FE2AC95B14581D20C0F|ribE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATTCCTGTAAACATGGTCA, downstream forward: _UP4_GGCTTTTAGAGAGGAAGATTReferences
Reviews
Original publications
12456892,15808508,11377200,8159171,7934830,7473709,23270261,24442413,26494285,29087189