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maeA
malic enzyme, required for malate dependent catabolite repression of the [gene|6F21B5E9EEF67E96808A1DEFA3A80DA2A0FF9462|pftA]-[gene|F6C3B267106E7510FABECF0B13ABF222901689A9|pftB] operon
function
malate utilization
product
NAD-dependent malate dehydrogenase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]Gene
Coordinates
3,800,418 3,802,166
The protein
Catalyzed reaction/ biological activity
(S)-malate + NAD+ --> CO2 + NADH + pyruvate (according to UniProt)H+ + oxaloacetate --> CO2 + pyruvate (according to UniProt)Protein family
[SW|malic enzymes family] (according to UniProt)Paralogous protein(s)
[protein|BBBCD5F56779895189E21076AF165B901F654534|MalS] [SW|Cofactors]
NAD+Structure
[PDB|1LLQ] (from Ascaris suum, 41% identity) [pubmed|12033925]Expression and Regulation
Operons
genes
[gene|4F30A4412768E6313F57BD56C4EF5079F500E0C4|maeA]-[gene|1E602BCB2A416CDBF2B5007765EC9628123FE979|ywkB]
description
[Pubmed|12949160]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12949160], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[protein|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|MalR]: activation, [Pubmed|12949160], in [regulon|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|MalR regulon]regulation
expressed in the presence of malate ([protein|search|MalR]) [Pubmed|12949160]additional information
the mRNA is substantially stabilized upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]view in new tabBiological materials
Mutant
MGNA-B657 (ywkA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1656 NBRP B. subtilis, Japan]GP1141 (aphA3) available in [SW|Jörg Stülke]'s labBKE37050 ([gene|4F30A4412768E6313F57BD56C4EF5079F500E0C4|maeA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAAAATATGACCGATC, downstream forward: _UP4_TAATTAGCGAGTAAATTTTTBKK37050 ([gene|4F30A4412768E6313F57BD56C4EF5079F500E0C4|maeA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAAAATATGACCGATC, downstream forward: _UP4_TAATTAGCGAGTAAATTTTTlabs
[SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, FranceReferences
21815947,23136871,12949160,16788182,28974613,12033925