SubtiBank SubtiBank
cwlO [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]
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cwlO [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

D,L-endopeptidase-type autolysin, primary autolytic pathway for cell elongation
locus
BSU34800
pI
5.00
mw
50.00 kDa
function
cell wall synthesis, cell elongation
product
endopeptidase-type autolysin
essential
no
synonyms
yzkA, yvcE

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.2|Autolytic activity required for peptidoglycan synthesis (cell elongation)]
  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.1|Autolysis]
  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.4|Endopeptidases]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
  • [SW|Categories] containing this gene/protein

  • [SW|cell wall synthesis], [SW|cell wall degradation/ turnover]
  • This gene is a member of the following [SW|regulons]

  • [SW|WalR regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    3,574,363 -> 3,575,784

    Phenotypes of a mutant

  • a ''[protein|search|cwlO] [protein|search|lytE]'' mutant is not viable [Pubmed|17581128,22139507]
  • shorter, fatter cells, this can be rescued by addition of Mg(2) [Pubmed|23869552,23855774]
  • The protein

    Catalyzed reaction/ biological activity

  • cleaves the peptide bond between D-Glu (position 2 in the peptioglycan peptide) and m-diamino pimelic acid (position 3) [Pubmed|18266855]
  • Protein family

  • peptidase C40 family (according to Swiss-Prot)
  • Paralogous protein(s)

  • the C-terminal D,L-endopeptidase domains of [protein|search|LytE], [protein|search|LytF], [protein|search|CwlS], and [protein|search|CwlO] exhibit strong sequence similarity
  • [SW|Domains]

  • C-terminal D,L-endopeptidase domain [Pubmed|22139507]
  • Effectors of protein activity

  • [protein|search|CwlO] requires activation by [protein|search|FtsE]-[protein|search|FtsX] [Pubmed|23869552,23855774]
  • activity requires functional [protein|search|Mbl] [Pubmed|23869552]
  • [SW|Localization]

  • extracellular (signal peptide) [Pubmed|18957862]
  • localizes to the outer lateral sidewall of the cell (via the N-terminal domain) [Pubmed|22139507]
  • cell membrane in a [protein|search|FtsX]-dependent manner [Pubmed|23869552]
  • [SW|Interactions]

  • [protein|search|FtsX]-[protein|search|CwlO], at the membrane [Pubmed|23869552]
  • Expression and Regulation

    [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|24163346]
  • Regulation

  • expressed during exponential growth ([protein|search|WalR]) [Pubmed|17581128]
  • Regulatory mechanism

  • [protein|search|WalR]: transcription activation [Pubmed|17581128]
  • Biological materials

    Mutant

  • BKE34800 (''[protein|search|cwlO]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s lab)
  • References

    Reviews

  • 23066944,18266855
  • Original publications

  • 21478646,16233686,17581128,20525796,18957862,20059685,22139507,23855774,23869552,24163346,27118079