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yutJ

putative NADH dehydrogenase

locus: BSU_32200

pI: 5.82

mw: 36.75 kDa

protein length: 355 aa

gene length: 1068 bp

function: unknown

product: putative NADH dehydrogenase

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.4|Electron transport/ other/ based on similarity]

Gene

Coordinates: 3,308,867 3,309,934

The protein

Protein family

• NADH dehydrogenase family (with [protein|56F407D408272F3674612C9CDFE4A922680EC694|Ndh] and [protein|9B3E9074975758BCB3D06820E4A35F8C2B78DCEB|YumB], according to UniProt)

[SW|Cofactors]

• FAD (according to UniProt) [Pubmed|21635694]

Structure

• [PDB|4XDB] (Ndh from ''S. aureus'', 24% identity)

Expression and Regulation

Operons

• genes: [gene|5044FDBEC8D81D1978DB431F9222902FCD80DD2D|yutJ]
  description: [Pubmed|22383849]

additional information

• the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]

Biological materials

Mutant

• MGNA-B575 (yutJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1574 NBRP B. subtilis, Japan]
• BKE32200 ([gene|5044FDBEC8D81D1978DB431F9222902FCD80DD2D|yutJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGAATTCCCCTTTAAT, downstream forward: _UP4_TAATGATAGAGAAGAACCGC
• BKK32200 ([gene|5044FDBEC8D81D1978DB431F9222902FCD80DD2D|yutJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGAATTCCCCTTTAAT, downstream forward: _UP4_TAATGATAGAGAAGAACCGC

References

• 17015645,26883633