SAVE THE DATE!
21st International Conference on Bacilli and Gram-Positive Bacteria. Prague, Czech Republic, June 7-11, 2021.
similar to oxygenase component of 4-hydroxyphenylacetate 3-monooxygenase
categories[category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 184.108.40.206|Newly identified sporulation proteins (based on transcription profiling)][category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]
Catalyzed reaction/ biological activity4-hydroxyphenylacetate + FADH2 + O2 --> 3,4-dihydroxyphenylacetate + FAD + H+ + H2O (according to UniProt)
Protein familyFADH(2)-utilizing monooxygenase family (single member, according to UniProt)
[SW|Cofactors]FAD (according to UniProt)
Structure[PDB|2YYG] (from ''Thermus thermophilus'', 42% identity) [Pubmed|17804419]
Expression and Regulation
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MutantMGNA-A838 (yoaI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/838 NBRP B. subtilis, Japan]BKE18620 ([gene|5224B7AD3B669120DBCC071540F6B37D11FD5260|yoaI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTAAGTTTCGCTCCTTC, downstream forward: _UP4_TGAAAAAACAGCGGGAAAAGBKK18620 ([gene|5224B7AD3B669120DBCC071540F6B37D11FD5260|yoaI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTAAGTTTCGCTCCTTC, downstream forward: _UP4_TGAAAAAACAGCGGGAAAAG