SubtiBank SubtiBank
pucR
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pucR

transcriptional regulator of puc genes
locus
BSU_32420
pI
6.19
mw
60.34 kDa
protein length
531 aa Sequence Blast
gene length
1596 bp Sequence Blast
function
regulation of purine utilization
product
transcriptional regulator of puc genes ([SW|PucR family])
essential
no
synonyms
yunI

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.1|Utilization of nucleotides]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
  • Gene

    Coordinates
    3,328,762 3,330,357

    The protein

    Protein family

  • [SW|PucR family]
  • [SW|CdaR family] (according to UniProt)
  • additional information

  • information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000058 PRODORIC2 database]
  • Expression and Regulation

    Operons

    genes
    [gene|52C1601482C26400A524E880334BB801F832D6ED|pucR]-[gene|53950BFDC128DE46F3F43BDB9D0FDB3D645152DF|pucJ]-[gene|3162EF36F4441A1E4EBBFDAD19F6768D8EF21B29|pucK]-[gene|2E7DEC53A130247E66225E87950D702E462C324F|pucL]-[gene|A2F93FFE225DFDBCBB3EA079BEF84C79465F0E5C|pucM]
    description
    [Pubmed|12823818]

    regulatory mechanism

  • [protein|52C1601482C26400A524E880334BB801F832D6ED|PucR]: auto-repression, [Pubmed|12029039], in [regulon|52C1601482C26400A524E880334BB801F832D6ED|PucR regulon]
  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: activation, [Pubmed|25755103], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]
  • regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|12823818]
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    Biological materials

    Mutant

  • MGNA-A934 (yunI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/934 NBRP B. subtilis, Japan]
  • BKE32420 ([gene|52C1601482C26400A524E880334BB801F832D6ED|pucR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGCGCATTCTCCTTTTC, downstream forward: _UP4_TAAAGTTTGTTACATTTTCT
  • BKK32420 ([gene|52C1601482C26400A524E880334BB801F832D6ED|pucR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGCGCATTCTCCTTTTC, downstream forward: _UP4_TAAAGTTTGTTACATTTTCT
  • References

  • 12374841,11344136,12029039,12823818,25755103