SAVE THE DATE! 21st International Conference on Bacilli and Gram-Positive Bacteria. Prague, Czech Republic, June 7-11, 2021.

ycbU

cysteine desulfurase

locus: BSU_02660

pI: 6.45

mw: 40.65 kDa

protein length: 370 aa

gene length: 1113 bp

function: unknown

product: cysteine desulfurase

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 287,499 288,611

The protein

Protein family

• [SW|Class-V pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

Paralogous protein(s)

• [protein|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|NifZ], [protein|88ACE6B79534338F7C72F107B35A0B2384007088|SufS], [protein|A6746AE1B92A0CDCCB22B57B6DF78094BB0223A8|NifS], [protein|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|YrvO]

[SW|Cofactors]

• PLP

Structure

• [PDB|5B87] (from Thermococcus onnurineus, corresponds to aa 4 ... 281, 26.5% identity) [pubmed|28536498]

Expression and Regulation

Operons

• genes: [gene|5424A936643C90B3DA2CB60FD0FD42A64F2BAF09|ycbU]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C039 (ycbU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2037 NBRP B. subtilis, Japan]
• BKE02660 ([gene|5424A936643C90B3DA2CB60FD0FD42A64F2BAF09|ycbU]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATGATTCACTCCTTTA, downstream forward: _UP4_TAAACATGAAAAAGCCCCTG
• BKK02660 ([gene|5424A936643C90B3DA2CB60FD0FD42A64F2BAF09|ycbU]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATGATTCACTCCTTTA, downstream forward: _UP4_TAAACATGAAAAAGCCCCTG

References

Research papers

• 28536498