yxjH

putative methionine synthase

locus: BSU_38950

pI: 5.09

mw: 38.14 kDa

protein length: 377 aa

gene length: 1134 bp

function: unknown

product: putative methionine synthase

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.11|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine/ based on similarity]

Gene

Coordinates: 3,997,964 3,999,097

The protein

Paralogous protein(s)

• [protein|A866ACE8431EC3FF98ADC734D9322AE699ECBFAD|YxjG]

Structure

• [PDB|1YPX] (the enzyme from ''Listeria monocytogenes'', 50% identity)

Expression and Regulation

Operons

• genes: [gene|55459D8F0BBB2669EB9F25276A17F1A7EB322AF1|yxjH]
  description: [Pubmed|10746760]

  regulatory mechanism

  • [regulon|S-box|S-box]: termination, the [SW|S-box] [SW|riboswitch] binds S-adenosylmethionine resulting in termination [Pubmed|10094622], in [regulon|S-box|S-box]

  regulation

  • induced by methionine starvation ([[SW|S-box]) [Pubmed|10094622]
  • the [SW|S-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

Biological materials

Mutant

• MGNA-B732 (yxjH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1731 NBRP B. subtilis, Japan]
• BKE38950 ([gene|55459D8F0BBB2669EB9F25276A17F1A7EB322AF1|yxjH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTATTCGCCTCTTTTC, downstream forward: _UP4_TAATCTATCATTGACAGAAA
• BKK38950 ([gene|55459D8F0BBB2669EB9F25276A17F1A7EB322AF1|yxjH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTATTCGCCTCTTTTC, downstream forward: _UP4_TAATCTATCATTGACAGAAA

References

• 19258532,10094622,18039762,12107147