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pucI
function
purine utilization
product
allantoin permease
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.6|Nucleotide/ nucleoside transporter][category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.1|Utilization of nucleotides][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]Gene
Coordinates
3,752,280 3,753,752
The protein
Catalyzed reaction/ biological activity
uptake of allantoin [Pubmed|26967546]Protein family
purine-cytosine permease (2.A.39) family (with [protein|CB969A2D60F106466605052299F2A5FF5A20E9ED|YxlA], according to UniProt)Structure
[PDB|2JLN] (from Microbacterium liquefaciens, 27% identity) [pubmed|18927357][SW|Localization]
cell membrane [Pubmed|26967546]Expression and Regulation
Operons
genes
[gene|5681A5F186AD4B8C81B78FD9DDAE2C0624BE7CAF|pucI]
description
[Pubmed|12029039]
regulatory mechanism
[protein|52C1601482C26400A524E880334BB801F832D6ED|PucR]: activation, [Pubmed|12029039], in [regulon|52C1601482C26400A524E880334BB801F832D6ED|PucR regulon]regulation
expression during spore [SW|germination] is strongly reduced under conditions of osmotic stress [Pubmed|27766092]view in new tabBiological materials
Mutant
MGNA-A897 (ywoE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/897 NBRP B. subtilis, Japan]BKE36470 ([gene|5681A5F186AD4B8C81B78FD9DDAE2C0624BE7CAF|pucI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGCCGGCTCCCCTTTCC, downstream forward: _UP4_TAAGATCTATTTAGACGGTABKK36470 ([gene|5681A5F186AD4B8C81B78FD9DDAE2C0624BE7CAF|pucI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGCCGGCTCCCCTTTCC, downstream forward: _UP4_TAAGATCTATTTAGACGGTAReferences
11344136,12029039,12029039,26967546,27766092,18927357