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function
lactate utilization
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]Gene
Coordinates
3,493,519 3,494,958
Phenotypes of a mutant
no growth with lactate as the single carbon source [Pubmed|19201793]The protein
Catalyzed reaction/ biological activity
oxidation of lactate to pyruvate [Pubmed|19201793]Protein family
LutB/YkgF family (single member, according to UniProt)[SW|Domains]
2 [SW|4Fe-4S ferredoxin-type domain]s (aa 304-334, aa 353-382) (according to UniProt)Modification
phosphorylated on Arg-33 [Pubmed|22517742][SW|Cofactors]
FeS clusterExpression and Regulation
Operons
genes
[gene|FEC83B66CEA311EA1650D61A215F82E9DF4E9F03|lutA]-[gene|57B9E37F6232189CD47C1B41FDCF43FCB8016AEB|lutB]-[gene|5EE69198882DF4E9AB8D9D7267F071EF88C2F16D|lutC]
description
[Pubmed|19201793]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|25031425], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, [Pubmed|19201793], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon][protein|E6F3BAC5875A16D8D75B2AD21447C14DD17B3DA6|LutR]: repression, in [regulon|E6F3BAC5875A16D8D75B2AD21447C14DD17B3DA6|LutR regulon]regulation
induction by lactate [Pubmed|19201793]view in new tabOther regulations
[protein|CE542AE1A32CCD1ABB259E6159F7A37A8078E189|FsrA]: translation repression, [Pubmed|22427629,18697947][protein|147AFEBEA546FDBEEB08E9A8D9C7BCDC9B83CC90|FbpB]: translation inhibition, acts as RNA chaperone, increases interaction between [protein|CE542AE1A32CCD1ABB259E6159F7A37A8078E189|FsrA] and [protein|FEC83B66CEA311EA1650D61A215F82E9DF4E9F03|LutA]-[protein|57B9E37F6232189CD47C1B41FDCF43FCB8016AEB|LutB]-[protein|5EE69198882DF4E9AB8D9D7267F071EF88C2F16D|LutC] RNAsBiological materials
Mutant
MGNA-A498 (yvfW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/498 NBRP B. subtilis, Japan]BKE34040 ([gene|57B9E37F6232189CD47C1B41FDCF43FCB8016AEB|lutB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATCCTTTCCCCCTCTG, downstream forward: _UP4_GAACGCACGAAGGAGGAGCABKK34040 ([gene|57B9E37F6232189CD47C1B41FDCF43FCB8016AEB|lutB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATCCTTTCCCCCTCTG, downstream forward: _UP4_GAACGCACGAAGGAGGAGCAlabs
[SW|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]References
22427629,18697947,16430695,22389480,19201793,19201793,22517742