SubtiBank SubtiBank
rnpA
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rnpA

protein component of RNase P, metal-dependent 5 end maturation of precursor tRNAs
locus
BSU_41050
pI
10.80
mw
13.66 kDa
protein length
116 aa Sequence Blast
gene length
351 bp Sequence Blast
function
cleavage of precursor sequences from the 5 ends of pre-tRNAs
product
protein component of RNase P (substrate specificity)
essential
yes
ec
3.1.26.5
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.2|Endoribonucleases]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    4,214,753 4,215,103

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor (according to UniProt)
  • 5' end maturation of tmRNA [Pubmed|25402410]

    • Protein family

  • RnpA family (single member, according to UniProt)

    • [SW|Domains]

  • RNR motif: residues 60-68, stabilizes complexes with both RNA part of [SW|RNase] P (''[gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB]'') and with pre-[gene|3B6620F44EC08BA9B02FE9359A5F74C6CAE6D4FB|trnA-Ile] ([gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB]-[protein|594F2D36BE71A45303A33AA2A2931F56F0A969ED|RnpA]-pre-[gene|3B6620F44EC08BA9B02FE9359A5F74C6CAE6D4FB|trnA-Ile]) [Pubmed|21622899]

    • Structure

  • [PDB|1A6F] [Pubmed|9563955]
  • [PDB|3Q1R] (complex of RnpA with RNA [protein|57A79AAF00243B5E058FA901D43AA7B55CA33F63|RnpB] and [gene|3B6620F44EC08BA9B02FE9359A5F74C6CAE6D4FB|trnA-Ile], from ''Thermotoga maritima'') [Pubmed|21076397]

    • Expression and Regulation

    Operons

    genes
    [gene|EB07AECD3849CC0B65126DE27E71B28B3AD106B0|rpmH]-[gene|594F2D36BE71A45303A33AA2A2931F56F0A969ED|rnpA]-[gene|D40C202E67A5319A91811DB11356F47A56C97DD2|yidC1]-[gene|23D33E2D7AB1CA02165070DB8238957E3C768E64|jag]
    description
    [Pubmed|22383849]
    view in new tab

    labs

  • [SW|Carol Fierke], University of Michigan, Ann Arbor, USA [https://www.chem.lsa.umich.edu/chem/faculty/facultyDetail.php?Uniqname=fierke homepage]
  • [SW|Roland Hartmann], Marburg University, Germany [http://www.pharmazie.uni-marburg.de/pharmchem/akhartmann/ag_hartmann.htm homepage]

    • References

    Reviews

  • 19215776,8901495,17868095,14691942,16650980,16980936,16679018,14550630,31464530

    • Original Publications

  • 10390342,17919279,17355991,11812129,16980484,11454206,19549719,11454206,9563955,19932118,17299131,20476778,11258888,16185070,21764917,22848659,21076397,20843005,21622899,24364358,25249623,25402410,26267651,27873254,31003868,31993626