rnpA

protein component of RNase P, metal-dependent 5 end maturation of precursor tRNAs

locus: BSU_41050

pI: 10.80

mw: 13.66 kDa

protein length: 116 aa

gene length: 351 bp

function: cleavage of precursor sequences from the 5 ends of pre-tRNAs

product: protein component of RNase P (substrate specificity)

essential: yes

ec: 3.1.26.5

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.2|Endoribonucleases]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 4,214,753 4,215,103

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor (according to UniProt)
• 5' end maturation of tmRNA [Pubmed|25402410]

Protein family

• RnpA family (single member, according to UniProt)

[SW|Domains]

• RNR motif: residues 60-68, stabilizes complexes with both RNA part of [SW|RNase] P (''[gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB]'') and with pre-[gene|3B6620F44EC08BA9B02FE9359A5F74C6CAE6D4FB|trnA-Ile] ([gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB]-[protein|594F2D36BE71A45303A33AA2A2931F56F0A969ED|RnpA]-pre-[gene|3B6620F44EC08BA9B02FE9359A5F74C6CAE6D4FB|trnA-Ile]) [Pubmed|21622899]

Structure

• [PDB|1A6F] [Pubmed|9563955]
• [PDB|3Q1R] (complex of RnpA with RNA [protein|57A79AAF00243B5E058FA901D43AA7B55CA33F63|RnpB] and [gene|3B6620F44EC08BA9B02FE9359A5F74C6CAE6D4FB|trnA-Ile], from ''Thermotoga maritima'') [Pubmed|21076397]

Expression and Regulation

Operons

• genes: [gene|EB07AECD3849CC0B65126DE27E71B28B3AD106B0|rpmH]-[gene|594F2D36BE71A45303A33AA2A2931F56F0A969ED|rnpA]-[gene|D40C202E67A5319A91811DB11356F47A56C97DD2|yidC1]-[gene|23D33E2D7AB1CA02165070DB8238957E3C768E64|jag]
  description: [Pubmed|22383849]

labs

• [SW|Carol Fierke], University of Michigan, Ann Arbor, USA [https://www.chem.lsa.umich.edu/chem/faculty/facultyDetail.php?Uniqname=fierke homepage]
• [SW|Roland Hartmann], Marburg University, Germany [http://www.pharmazie.uni-marburg.de/pharmchem/akhartmann/ag_hartmann.htm homepage]

References

Reviews

• 19215776,8901495,17868095,14691942,16650980,16980936,16679018,14550630,31464530

Original Publications

• 10390342,17919279,17355991,11812129,16980484,11454206,19549719,11454206,9563955,19932118,17299131,20476778,11258888,16185070,21764917,22848659,21076397,20843005,21622899,24364358,25249623,25402410,26267651,27873254,31003868,31993626