SubtiBank SubtiBank
rnpA
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

rnpA

protein component of RNase P, metal-dependent 5 end maturation of precursor tRNAs
locus
BSU41050
pI
10.80
mw
13.66 kDa
protein length
116 aa Sequence Blast
gene length
348 bp Sequence Blast
function
cleavage of precursor sequences from the 5 ends of pre-tRNAs
product
protein component of RNase P (substrate specificity)
essential
yes
ec
3.1.26.5
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.2|Endoribonucleases]

    • Gene

    Coordinates
    4,214,753 → 4,215,103

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from [gene|3B6620F44EC08BA9B02FE9359A5F74C6CAE6D4FB|trnA-Ile] precursor (according to Swiss-Prot)
  • 5' end maturation of tmRNA [Pubmed|25402410]

    • Protein family

  • rnpA family (according to Swiss-Prot)

    • [SW|Domains]

  • RNR motif: residues 60-68, stabilizes complexes with both the RNA part of [SW|RNase] P (''[gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB]'') and with pre-[gene|3B6620F44EC08BA9B02FE9359A5F74C6CAE6D4FB|trnA-Ile] ([gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB]-[protein|594F2D36BE71A45303A33AA2A2931F56F0A969ED|RnpA]-pre-[gene|3B6620F44EC08BA9B02FE9359A5F74C6CAE6D4FB|trnA-Ile]) [Pubmed|21622899]

    • Structure

  • [PDB|1A6F] [Pubmed|9563955]
  • [PDB|3Q1R] (complex of RnpA with the RNA [protein|57A79AAF00243B5E058FA901D43AA7B55CA33F63|RnpB] and [gene|3B6620F44EC08BA9B02FE9359A5F74C6CAE6D4FB|trnA-Ile], from ''Thermotoga maritima'') [Pubmed|21076397]

    • Labs working on this gene/protein

  • [SW|Carol Fierke], University of Michigan, Ann Arbor, USA [https://www.chem.lsa.umich.edu/chem/faculty/facultyDetail.php?Uniqname=fierke homepage]
  • [SW|Roland Hartmann], Marburg University, Germany [http://www.pharmazie.uni-marburg.de/pharmchem/akhartmann/ag_hartmann.htm homepage]

    • References

    Reviews

  • 19215776,8901495,17868095,14691942,16650980,16980936,16679018,14550630

    • Original Publications

  • 10390342,17919279,17355991,11812129,16980484,11454206,19549719,11454206,9563955,19932118,17299131,20476778,11258888,16185070,21764917,22848659,21076397,20843005,21622899,24364358,25249623,25402410,26267651,27873254