prfA

peptide chain release factor 1

locus: BSU_37010

pI: 4.82

mw: 40.07 kDa

protein length: 356 aa

gene length: 1071 bp

function: translation

product: peptide chain release factor 1

essential: yes

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.11|Translation factors]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 3,797,085 3,798,155

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• binds to the ribosome through specific recognition of messenger RNA stop codons and triggers hydrolysis of the bond between the nascent polypeptide and the transfer RNA at the peptidyl-tRNA site, thereby releasing the newly synthesized protein. PrfA is highly specific for a U in the first stop-codon position and recognizes UAA and UAG

Protein family

• prokaryotic/mitochondrial release factor family (with [protein|12E06FD3A36D95AE82E3C65788FA98178039592F|PrfB], according to UniProt)

Structure

• [PDB|1ZBT] (from ''Streptococcus mutans'', 58% identity)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|59E6468FD891F5271F38543515867B0B4625768D|prfA]-[gene|CF959D90A9B8F83E40E7B81CBFB00B5EC09EC0DD|prmC]-[gene|04C5876C267D77E59F57A3E7EA19C36FD35A03EF|ywkF]
  description: [Pubmed|22383849]

• genes: [gene|59E6468FD891F5271F38543515867B0B4625768D|prfA]
  description: [Pubmed|22383849]

References

• 12237459,20512119,25217634