uxaC

glucuronate isomerase (D-glucuronate, D-galacturonate)

locus: BSU_12300

pI: 5.17

mw: 54.44 kDa

protein length: 473 aa

gene length: 1422 bp

function: hexuronate utilization

product: glucuronate isomerase (D-glucuronate, D-galacturonate)

essential: no

ec: 5.3.1.12

synonyms: yjmA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.15|Utilization of hexuronate]

Gene

Coordinates: 1,300,450 1,301,871

The protein

Catalyzed reaction/ biological activity

• D-glucuronate --> D-fructuronate (according to UniProt)
• aldehydo-D-galacturonate --> keto-D-tagaturonate (according to UniProt)

Protein family

• [SW|metallo-dependent hydrolases superfamily] (according to UniProt)

Structure

• [PDB|3IAC] (from Salmonella typhimurium, 49% identity)

Expression and Regulation

Operons

• genes: [gene|5A0C29C7E218701727523885D01A48D02266B421|uxaC]-[gene|59CD3E4C24326542D9C85BB6FE64FBF49869EB55|yjmB]-[gene|3AEA5D769B89E676C916FD8934D762B4C9A02AA2|yjmC]-[gene|CC55984E31C5698668CCCD4AC6E2210B2B41ACAB|yjmD]-[gene|A0A6D36BEC397345A1D17E6A7C34E1F612FD46C7|uxuA]-[gene|B886E504A767343042DE9BF4E23E6798D124A2CF|yjmF]-[gene|6D08E897DD697523FD0720704A25AD4472075B6C|exuT]-[gene|69B143987DCF73B5D080E3FD87A6CA2940E07DA8|exuR]-[gene|A1C64447E0074B06C9210CC51680273FAAE703FA|uxaB]-[gene|ED29D2DE48AADEA09C90F08B6021555C357946B4|uxaA]
  description: [Pubmed|9882655]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9882655], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|69B143987DCF73B5D080E3FD87A6CA2940E07DA8|ExuR]: repression, in the absence of inducer, in [regulon|69B143987DCF73B5D080E3FD87A6CA2940E07DA8|ExuR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [pubmed|9882655] [pubmed|10666464], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • induced by galacturonate ([protein|search|ExuR]) [Pubmed|9882655]

Biological materials

Mutant

• MGNA-A365 (yjmA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/365 NBRP B. subtilis, Japan]
• BKE12300 ([gene|5A0C29C7E218701727523885D01A48D02266B421|uxaC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCTCACCGTCTTTCA, downstream forward: _UP4_TAACAAAGTGTCCGCTCAGT
• BKK12300 ([gene|5A0C29C7E218701727523885D01A48D02266B421|uxaC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCTCACCGTCTTTCA, downstream forward: _UP4_TAACAAAGTGTCCGCTCAGT

References

• 9579062,22900538,9579062,9882655,10666464