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cysJ
sulfite reductase (NADPH2) (alpha subunit)
function
sulfite reduction
product
sulfite reductase (NADPH2)
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism][category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism] → [category|SW 2.6.4.2|sulfur metabolism/ general]Gene
Coordinates
3,432,339 3,434,156
Phenotypes of a mutant
unable to grow with sulfate or sulfite as the only sulfur source [Pubmed|12169591]defective in the the ability to support the growth of ''Synechococcus leopoliensis'' CCAP1405/1 on agar media [Pubmed|25875741]The protein
Catalyzed reaction/ biological activity
3 H2O + hydrogen sulfide + 3 NADP+ --> 4 H+ + 3 NADPH + sulfite (according to UniProt)[SW|Domains]
[SW|Flavodoxin-like domain] (aa 68-206) (according to UniProt)[SW|FAD-binding FR-type domain] (aa 235-454) (according to UniProt)[SW|Cofactors]
FMN [Pubmed|21635694]FAD [Pubmed|21635694]Structure
[PDB|5GXU] (from Arabidopsis, 32% identity)additional information
belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]Expression and Regulation
Operons
genes
[gene|A4C9E4D1163B703A917BFCF97A5F575B19EDDD8D|cysI]-[gene|5A99F57DF0DDF5A129C3702E97621232A8173C22|cysJ]
description
[Pubmed|12169591]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12169591], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[protein|FB37E9B7F7727915ADB18E52FD07A8773C7B30FC|CysL]: activation, [Pubmed|12169591], in [regulon|FB37E9B7F7727915ADB18E52FD07A8773C7B30FC|CysL regulon]regulation
induced by sulfite, sulfate, and thiosulfate ([protein|search|CysL]) [Pubmed|12169591]view in new tabBiological materials
Mutant
1A802 ( ''cysJ''::''kan''), [Pubmed|11445163], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A802&Search=1A802 BGSC]1A934 ( ''cysJ''::''kan''), [Pubmed|12169591], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A934&Search=1A934 BGSC]BKE33440 ([gene|5A99F57DF0DDF5A129C3702E97621232A8173C22|cysJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACGTATCCACCTCACAAA, downstream forward: _UP4_TGATTTGACTTGAAAGGAGTBKK33440 ([gene|5A99F57DF0DDF5A129C3702E97621232A8173C22|cysJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACGTATCCACCTCACAAA, downstream forward: _UP4_TGATTTGACTTGAAAGGAGTReferences
15378759,12169591,12107147,11445163,25875741