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mtnN
methylthioadenosine nucleosidase
function
methionine salvage
product
methylthioadenosine nucleosidase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.10|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine]Gene
Coordinates
2,787,130 2,787,825
The protein
Catalyzed reaction/ biological activity
H2O + S-adenosyl-L-homocysteine --> adenine + S-(5-deoxy-D-ribos-5-yl)-L-homocysteine (according to UniProt)H2O + S-methyl-5'-thioadenosine --> adenine + S-methyl-5-thio-D-ribose (according to UniProt)Protein family
PNP/UDP phosphorylase family (with [protein|3075327DBE55C4F628171AEE248CAED5870693FA|DeoD], according to UniProt)Structure
[PDB|1JYS] (from ''Escherichia coli'', 51% identity, 70% similarity) [Pubmed|11591349]Expression and Regulation
Operons
genes
[gene|992A24CF9492965BF0E9EEFBB6F0A06871275068|yrrT]-[gene|5B21D1E73A65B13CE4DDA32ABC71EFA54E8D1D8F|mtnN]-[gene|F195C47849F4F6B4C56E3A386F5D8C84326B01C4|mccA]-[gene|314CB4923D15140E72B0ABAFE3E8BE9CA7310E10|mccB]-[gene|177EFFDCA6E3C3BB840EED13D9F2D71F99B833B6|yrhC]
description
[Pubmed|17056751]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16513748], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16513748], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon][protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|12642660,16885442], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]regulation
repressed in the presence of cysteine ([protein|search|CymR]) [Pubmed|16513748]view in new tabBiological materials
Mutant
MGNA-A850 (yrrU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/850 NBRP B. subtilis, Japan]BKE27270 ([gene|5B21D1E73A65B13CE4DDA32ABC71EFA54E8D1D8F|mtnN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATTCACATTCCCTTC, downstream forward: _UP4_TAAAACGGGGAGAACGTTCTBKK27270 ([gene|5B21D1E73A65B13CE4DDA32ABC71EFA54E8D1D8F|mtnN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATTCACATTCCCTTC, downstream forward: _UP4_TAAAACGGGGAGAACGTTCTReferences
17056751,16513748,12642660,15102328,16885442,24509311