SubtiBank SubtiBank
dnaC
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dnaC

replicative DNA helicase, part of the [SW|replisome]
locus
BSU_40440
pI
4.55
mw
50.44 kDa
protein length
454 aa Sequence Blast
gene length
1365 bp Sequence Blast
function
[category|SW 3.1.1|DNA replication]
product
replicative DNA helicase
essential
yes
ec
3.6.4.12
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    4,157,471 4,158,835

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • stimulates [protein|F7B53E8AC92DAD15544C6208C2A5B41BABA73D6D|DnaG] primase activity [Pubmed|23563155]
  • ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
  • Protein family

  • [SW|helicase family] (according to UniProt)
  • [SW|Domains]

  • SF4 helicase domain (aa 179-445) (according to UniProt)
  • Structure

  • [PDB|2R6A] (Geobacillus stearothermophilus), [PDB|2R6C], [PDB|2R6D], [PDB|2R6E]
  • [PDB|4M4W] (the [protein|5CF09E089AA70A32F640CE0D33D1F05312AAE24D|DnaC]-[protein|AC1B507C6B1640E9BEA1F9F01628120F3680C976|DnaI]-[protein|F7B53E8AC92DAD15544C6208C2A5B41BABA73D6D|DnaG] complex) [Pubmed|24048025]
  • [SW|Localization]

  • Cytoplasm (Homogeneous) [Pubmed|16479537]
  • Expression and Regulation

    Operons

    genes
    [gene|5CF09E089AA70A32F640CE0D33D1F05312AAE24D|dnaC]
    description
    [pubmed|22383849]
    view in new tab

    Biological materials

    two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab
  • References

    Reviews

  • 22797751,28075389
  • Original publications

  • 10844689,12718886,11250911,20122408,16479537,10844689,19744498,19968790,12060778,23525462,23563155,24048025,30285297