pel

pectate lyase C

locus: BSU_07560

pI: 8.42

mw: 45.34 kDa

protein length: 420 aa

gene length: 1263 bp

function: degradation of polygalacturonic acid

product: pectate lyase C

essential: no

ec: 4.2.2.2

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.27|Utilization of other polymeric carbohydrates]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 827,993 829,255

The protein

Catalyzed reaction/ biological activity

• Eliminative cleavage of (14)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends (according to UniProt)

Protein family

• [SW|lyase 1 family] (according to UniProt)

Structure

• [PDB|2O1D] (bound to trisaccharide), [PDB|1BN8], [PDB|5X2I]

[SW|Localization]

• extracellular (signal peptide), major constituent of the secretome [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|60595234B02D673E860B364FE8A3C83D2CC68CB2|pel]
  description: [Pubmed|12823818]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: repression, [Pubmed|12823818], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]
  • [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA]: activation, ([protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA]) [Pubmed|16091051], in [regulon|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA regulon]

  regulation

  • expressed at high cell density ([protein|search|ComA]) [Pubmed|16091051]

Biological materials

Mutant

• MGNA-C248 (pel::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2246 NBRP B. subtilis, Japan]
• BKE07560 ([gene|60595234B02D673E860B364FE8A3C83D2CC68CB2|pel]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCACTATGTACCCCCAT, downstream forward: _UP4_TAAGAAAGTGAAAAACACAA
• BKK07560 ([gene|60595234B02D673E860B364FE8A3C83D2CC68CB2|pel]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCACTATGTACCCCCAT, downstream forward: _UP4_TAAGAAAGTGAAAAACACAA

References

Reviews

• 20735481

Original publications

• 8262178,16091051,12823818,18957862,12850135,25755103,7634076,26582911,27447541,27965289