SubtiBank SubtiBank
ptkA
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

ptkA

protein tyrosine kinase
locus
BSU36250
pI
9.63
mw
25.64 kDa
protein length
237 aa Sequence Blast
gene length
711 bp Sequence Blast
function
protein phosphorylation
product
protein tyrosine kinase
essential
no
synonyms
ywqD

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.7|Phosphorylation on a Tyr residue]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.4|Regulation]
  • Gene

    Coordinates
    3,731,822 → 3,732,535

    Phenotypes of a mutant

  • Accumulation of extra chromosome equivalents [Pubmed|17367396]
  • Defect in [SW|biofilm formation], this involves the kinase activity, but the target protein is unknown [Pubmed|20815827]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate (according to Swiss-Prot), autophosphorylation, phosphorylation of [protein|45AFD434F5262BFBE4ED6DF3D18DAED61FF317D1|Ugd], [protein|DF8CD0B3997A7A4CF4821A7D64E9898422FD17E0|TuaD], [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|SsbB], [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|SsbB]
  • Protein family

  • BY-kinase, see the [http://bykdb.ibcp.fr/BYKdb/BYKdbHelp Bacterial Protein Tyrosine Kinase Database])
  • Paralogous protein(s)

  • [protein|9B668909E1380B287ACE58561DCF45FC29184D8B|EpsB]
  • [SW|Domains]

  • single BY-kinase domain
  • Modification

  • autophosphorylation at residues Y225, Y227 and Y228 (primary site) [Pubmed|20509597], dephosphorylated by [protein|CF73D86DB024575BE8F043A4C3996458F4262E46|PtpZ] [Pubmed|15866923]
  • [SW|Cofactors]

  • ATP
  • Effectors of protein activity

  • [protein|8DCA50478136628DD9E5D01E89609A686EED9F57|TkmA] - transmembrane modulator, activates PtkA autophosphorylation and substrate phosphorylation [Pubmed|12970183]
  • the mutually exclusive interactions of PtsA with the modulator proteins [protein|8DCA50478136628DD9E5D01E89609A686EED9F57|TkmA], [protein|8841E35B7A90F6716C0BCD98E8D0CC05FE1CA471|SalA], and probably [protein|37DAD42A391E2FC506225EAF91B8F21629A401DF|MinD] direct the kinase to different substrates [Pubmed|27725816]
  • Structure

  • [PDB|2VED] (CapB, the homolog in ''Staphylococcus aureus'')
  • Expression and Regulation

    Operons

    genes
    [gene|8DCA50478136628DD9E5D01E89609A686EED9F57|tkmA]-[gene|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]-[gene|CF73D86DB024575BE8F043A4C3996458F4262E46|ptpZ]-[gene|45AFD434F5262BFBE4ED6DF3D18DAED61FF317D1|ugd]
    description
    [Pubmed|20815827,12970183]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|20815827], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [Pubmed|26283769], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P, [Pubmed|26283769], in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
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    Biological materials

    Mutant

  • MGNA-A078 (ywqD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/78 NBRP B. subtilis, Japan]
  • KO strain created with pMUTIN-2, available from [SW|Ivan Mijakovic]
  • GP1520 (spc), available in [SW| Jörg Stülke]'s lab
  • GP1544 (ermC), available in [SW| Jörg Stülke]'s lab
  • GP1587 (cat) , available in [SW| Jörg Stülke]'s lab
  • GP1521 ''[gene|9B668909E1380B287ACE58561DCF45FC29184D8B|epsB]'' (aphA3) ''[gene|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]'' (spc) double mutant available in [SW| Jörg Stülke]'s lab
  • GP1529 ''[gene|8DCA50478136628DD9E5D01E89609A686EED9F57|tkmA]-[gene|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]''::spc available in [SW| Jörg Stülke]'s lab
  • GP1610 (''[gene|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]-[gene|CF73D86DB024575BE8F043A4C3996458F4262E46|ptpZ]'', spc), available in [SW| Jörg Stülke]'s lab
  • BKE36250 (Δ[gene|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]::erm trpC2), available at [http://www.bgsc.org/getdetail.php?bgscid=BSU36250 BGSC], upstream reverse: _UP1_CTGCATCCGCGAGCCTCTGT, downstream forward: _UP4_TAAATAACGTGCATCGTGCC
  • BKK36250 (Δ[gene|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]::kan trpC2), available at [http://www.bgsc.org/getdetail.php?bgscid=BSU36250 BGSC], upstream reverse: _UP1_CTGCATCCGCGAGCCTCTGT, downstream forward: _UP4_TAAATAACGTGCATCGTGCC
  • Expression vector

  • pQE-30, N-terminally 6xHis-tagged, available from [SW|Ivan Mijakovic]
  • lacZ fusion

  • in a KO strain created with pMUTIN-2, available from [SW|Ivan Mijakovic]
  • GFP fusion

  • CFP-fusion, available from [SW|Ivan Mijakovic]
  • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW| Jörg Stülke]'s lab
  • Labs working on this gene/protein

  • [SW|Ivan Mijakovic], Thiverval-Grignon, France
  • References

    Reviews

  • 20497498,24554699,25540643,25667587,26286503
  • Original publications

  • 12970183,15741737,15866923,17367396,19258708,18547145,20497499,20509597,20815827,20817675,23939619,24493247,24728941,25278935,25374563,26283769,26094643,27148221,27725816