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rpsNB

alternative ribosomal protein S14*, can replace RpsN under conditions of zinc limitation

locus: BSU08880

pI: 11.19

mw: 10.20 kDa

protein length: 89 aa

gene length: 267 bp

function: translation

product: alternative ribosomal protein S14*

essential: no

synonyms: rpsNB,yhzA

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.4|Ribosomal proteins]

Gene

Coordinates: 965,909 → 966,178

The protein

Paralogous protein(s)

• [protein|0CC562884650F322298BEA28C889E2ABCB01AFDE|RpsN]

Expression and Regulation

Operons

• genes: [gene|6212D2DB719ACC477DA835EAC198545318FFEFAC|rpsNB]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur]: repression, in the presence of zinc [PubMed|12904577,17163968], in [regulon|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur regulon]

  regulation

  • induced by zinc starvation ([protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur]) [PubMed|12904577,17163968]
  • part of the third wave of induction upon zinc depletion [Pubmed|27561249]

additional information

• the mRNA is very stable (> 15 min) [pubmed|12884008]

Biological materials

Mutant

• BKE08880 (Δ[gene|6212D2DB719ACC477DA835EAC198545318FFEFAC|rpsNB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGTCACATAGCCTCCCTT, downstream forward: _UP4_TGGTAAGAAAAAACTCCGGC
• BKK08880 (Δ[gene|6212D2DB719ACC477DA835EAC198545318FFEFAC|rpsNB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGTCACATAGCCTCCCTT, downstream forward: _UP4_TGGTAAGAAAAAACTCCGGC

References

• 17163968,12884008,12904577,20208344,12904577,17163968,19648245,23002217,27561249