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rpsNB
alternative ribosomal protein S14*, can replace [protein|0CC562884650F322298BEA28C889E2ABCB01AFDE|RpsN] under conditions of zinc limitation
product
alternative ribosomal protein S14*
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.4|Ribosomal proteins]Gene
Coordinates
965,909 966,178
The protein
Protein family
universal [SW|ribosomal protein] uS14 family (with [protein|0CC562884650F322298BEA28C889E2ABCB01AFDE|RpsN], according to UniProt)Paralogous protein(s)
[protein|0CC562884650F322298BEA28C889E2ABCB01AFDE|RpsN] Structure
[PDB|5AFI] (the E. coli ribosome, 47% identity) [pubmed|25707802]Expression and Regulation
Operons
genes
[gene|6212D2DB719ACC477DA835EAC198545318FFEFAC|rpsNB]
description
[pubmed|22383849]
regulatory mechanism
[protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur]: repression, in the presence of zinc [PubMed|12904577,17163968], in [regulon|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur regulon][protein|F4097349A563503468A2A14F062AEAC532C7917A|YlxR]: regulation, [pubmed|30355672], in [regulon|F4097349A563503468A2A14F062AEAC532C7917A|YlxR regulon]regulation
induced by zinc starvation ([protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur]) [PubMed|12904577,17163968]part of the third wave of induction upon zinc depletion [Pubmed|27561249]expression requires functional [protein|F4097349A563503468A2A14F062AEAC532C7917A|YlxR] [pubmed|30355672]view in new tabadditional information
the mRNA is very stable (> 15 min) [pubmed|12884008]Biological materials
Mutant
BKE08880 ([gene|6212D2DB719ACC477DA835EAC198545318FFEFAC|rpsNB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGTCACATAGCCTCCCTT, downstream forward: _UP4_TGGTAAGAAAAAACTCCGGCBKK08880 ([gene|6212D2DB719ACC477DA835EAC198545318FFEFAC|rpsNB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGTCACATAGCCTCCCTT, downstream forward: _UP4_TGGTAAGAAAAAACTCCGGCReferences
17163968,12884008,12904577,20208344,12904577,17163968,19648245,23002217,27561249,25707802