SubtiBank SubtiBank
phoP

phoP

two-component response regulator (OmpR family), regulation of phosphate metabolism
locus
BSU29110
pI
5.07
mw
27.53 kDa
protein length
240 aa Sequence Blast
gene length
720 bp Sequence Blast
function
regulation of phosphate metabolism (phoA, phoB, phoD, resA | resABCDE, tagA-tagB, tagD | tagDEF, tuaA | tuaA-H)
product
two-component response regulator (OmpR family)
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.3|Phosphate metabolism]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.1|Two-component system response regulators]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.5|Regulators of core metabolism]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.2|Phosphorylation on an Asp residue]
  • Gene

    Coordinates
    2,977,800 → 2,978,522

    The protein

    Protein family

  • [SW|OmpR family] of two-component response regulators
  • Modification

  • phosphorylation by [protein|C81F68521F17861D50926D7D8757DC1D2340BC0D|PhoR] under conditions of phosphate limitation (stimulates DNA-binding activity)
  • Effectors of protein activity

  • phosphorylation stimulates DNA-binding activity
  • Structure

  • [PDB|1MVO] (receiver domain)
  • [SW|Localization]

  • cell membrane (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]-[gene|C81F68521F17861D50926D7D8757DC1D2340BC0D|phoR]
    description
    [Pubmed|14762014]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16452408], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15205429], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]
  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190,15205429], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
  • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|16452408,12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC]: repression, [Pubmed|20382764], in [regulon|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC regulon]
  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, due to [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]~P binding to the 3’end of the [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]-[protein|C81F68521F17861D50926D7D8757DC1D2340BC0D|PhoR] operon [Pubmed|25666134,15205429,14762014], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]
  • regulation

  • carbon catabolite repression ([protein|search|CcpA]) [Pubmed|16452408,12850135]
  • view in new tab

    Biological materials

    Mutant

  • 1A966 ( ''phoP''::''tet''), [Pubmed|14973033], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A966&Search=1A966 BGSC]
  • Labs working on this gene/protein

  • [SW|Marion Hulett], University of Illinois at Chicago, USA [http://www.uic.edu/depts/bios/faculty/hulett/hulettm.shtml Homepage]
  • References

    Regulation of ''[gene|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]]-[[phoR]'' expression

  • 20382764,14762014,12850135,15205429,16452408,25666134
  • Biochemical analyses

  • 9680208,9611818,12486063,20167622,10433720,17085571,14973033,12486062
  • Targets of PhoR==

  • Additional publications:''' [Pubmed|21636651]
  • 20059685 16030210, 10913081,10094677,9683503,9988472,9457886,9335276,9593301,9098050,
  • Other original publications

  • 10094672,15576792