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yojF

unknown

locus: BSU_19470

pI: 7.32

mw: 12.90 kDa

protein length: 116 aa

gene length: 351 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 2,122,325 2,122,675

The protein

Structure

• [PDB|1NJH]

Expression and Regulation

Operons

• genes: [gene|7922A49FBC7CCDC8C585EF6E67D7CC9CC91059F7|yoyC]-[gene|63EC848DB7CC4464B070E65D2C9288851B08BBE3|yojF]-[gene|8106404D9D2CFA0825E1284D47CC7198D8A20E5D|bshB2]
  description: (major transcript) [Pubmed|23894131,20308541]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|23894131], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|23894131], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

  regulation

  • ''[protein|search|yoyC]'': induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]

• genes: [gene|FBF1A8C92149A216D8A46F9E02898D7C5C448E2A|yojE]-[gene|7922A49FBC7CCDC8C585EF6E67D7CC9CC91059F7|yoyC]-[gene|63EC848DB7CC4464B070E65D2C9288851B08BBE3|yojF]-[gene|8106404D9D2CFA0825E1284D47CC7198D8A20E5D|bshB2]
  description: [Pubmed|23894131]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|23894131], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulation

  • ''[protein|search|yoyC]'': induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]

Biological materials

Mutant

• MGNA-B429 (yojF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1428 NBRP B. subtilis, Japan]
• CS211 (''[gene|63EC848DB7CC4464B070E65D2C9288851B08BBE3|yojF]''::''aphA3'', available in [SW|Colin Harwood]'s and [SW|Jörg Stülke]'s labs) [Pubmed|27197833]
• BKE19470 ([gene|63EC848DB7CC4464B070E65D2C9288851B08BBE3|yojF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATGATTGCTCCCCTCT, downstream forward: _UP4_GTTTAAAGAAAAGGGTGAAT
• BKK19470 ([gene|63EC848DB7CC4464B070E65D2C9288851B08BBE3|yojF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATGATTGCTCCCCTCT, downstream forward: _UP4_GTTTAAAGAAAAGGGTGAAT

References

Operon and expression

• 20308541,23894131,27197833