SubtiBank SubtiBank
dnaE
The second international online conference #Subtillery2021 will be held 14th - 18th June - save the date, for more information see the conference website!

The 21st International Conference on Bacilli has been postponed to 2022 and will take place in Prague.

dnaE

lagging strand DNA polymerase III (alpha subunit), part of the [SW|replisome]
locus
BSU_29230
pI
6.02
mw
125.14 kDa
protein length
1115 aa Sequence Blast
gene length
3348 bp Sequence Blast
function
[category|SW 3.1.1|DNA replication](lagging strand)
product
lagging strand DNA polymerase III (alpha subunit)
essential
yes
ec
2.7.7.7
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    2,991,269 2,994,616

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) --> diphosphate + DNA(n+1) (according to UniProt)
  • required for bacteriophage SPP1 replication [Pubmed|23268446]
  • Protein family

  • DNA polymerase type-C family (with [protein|48BC0FA9901F174472EB070C539EC75EC7C10E88|YorL] and [protein|FB97DF0C147FB29944F60214CB9BC1803861DAA0|PolC], according to UniProt)
  • Structure

  • [PDB|3E0D] (from ''Thermus aquaticus'', 39% identity) [Pubmed|18691598]
  • [SW|Localization]

  • forms foci, the foci dissappear after the formation of DNA mismatches in a [protein|E2A8B04CD729332F9E6A13195181983F751F2F25|MutS]-dependent manner or after DNA replication arrest [Pubmed|21958350]
  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|65B5EA4DBDF5B260DED754B8A7B226AA88F17B55|dnaE]-[gene|45229A538D5124B60CE05DDC8699B9BC3D5AD9FF|ytsJ]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [PubMed|14593098,16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]
  • regulation

  • induced by DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14593098,16267290 PubMed]
  • view in new tab

    References

    Reviews

  • 22933559,28073967
  • Original publications

  • 11721055,14593098,16267290,20122408,23563155,23017159,21958350,23268446,24062730,24106089,18691598,28575448,28878042,30686488,31251806