The second international online conference #Subtillery2021 will be held 14th - 18th June - save the date, for more information see the conference website!

The 21st International Conference on Bacilli has been postponed to 2022 and will take place in Prague.

ywjB

unknown

locus: BSU_37220

pI: 4.53

mw: 19.44 kDa

protein length: 174 aa

gene length: 525 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 3,819,220 3,819,744

The protein

Structure

• [PDB|2GD9] ([protein|E2CABD337831904871E0F0260DAA992E41A4F9E0|YyaP], 31% identity)

[SW|Localization]

• membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|07752EC6940F43DEBD1E9313A2A195E35EDB9F69|ywiE]-[gene|D5A3C316BD4567A0155F70330E32ACDE18F063FE|ywjA]-[gene|685B58AD2A02A0B48B7D4AAAE046DE2CF8F77C58|ywjB]
  description: [pubmed|22383849]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|26577401], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

• genes: [gene|D5A3C316BD4567A0155F70330E32ACDE18F063FE|ywjA]-[gene|685B58AD2A02A0B48B7D4AAAE046DE2CF8F77C58|ywjB]
  description: [pubmed|22383849]

• genes: [gene|685B58AD2A02A0B48B7D4AAAE046DE2CF8F77C58|ywjB]
  description: [Pubmed|9353933]

  regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

  regulation

  • repressed unless the cells enter an iron starvation ([protein|search|Fur]) [Pubmed|12354229]

Biological materials

Mutant

• MGNA-B661 (ywjB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1660 NBRP B. subtilis, Japan]
• BKE37220 ([gene|685B58AD2A02A0B48B7D4AAAE046DE2CF8F77C58|ywjB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCTCCTCCTATCGAT, downstream forward: _UP4_TAAGAAAAAGGATAGACAGA
• BKK37220 ([gene|685B58AD2A02A0B48B7D4AAAE046DE2CF8F77C58|ywjB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCTCCTCCTATCGAT, downstream forward: _UP4_TAAGAAAAAGGATAGACAGA

References

• 12354229,9353933