malR

two-component response regulator, regulation of malate uptake

locus: BSU_31530

pI: 5.98

mw: 26.82 kDa

protein length: 235 aa

gene length: 708 bp

function: regulation of malate uptake

product: two-component response regulator

essential: no

synonyms: yufM

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.1|Two-component system response regulators]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.2|Phosphorylation on an Asp residue]

Gene

Coordinates: 3,238,751 3,239,458

Phenotypes of a mutant

• unable to use malate as the single carbon source [Pubmed|12949159]

The protein

[SW|Domains]

• [SW|Response regulatory domain] (aa 3-119) (according to UniProt)

Modification

• phosphorylated by [protein|6FBBC2BDEAD72BEA0D9EA56668FDA5A3BE6F694E|MalK] on an Asp residue

Effectors of protein activity

• phosphorylation likely affects DNA-binding activity

Structure

• [PDB|5LWK] (from Lactobacillus paracasei, N-terminal part, corresponds to aa 1 ... 122, 43% identity) [pubmed|28577341]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|malR]
  description: [Pubmed|9274030]

• genes: [gene|6FBBC2BDEAD72BEA0D9EA56668FDA5A3BE6F694E|malK]-[gene|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|malR]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A635 (yufM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/635 NBRP B. subtilis, Japan]
• GP1301 (cat), available in [SW|Jörg Stülke]'s lab
• BKE31530 ([gene|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|malR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAATCATGATTTTCCTC, downstream forward: _UP4_TAAGAAAACGCACTGCTTGC
• BKK31530 ([gene|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|malR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAATCATGATTTTCCTC, downstream forward: _UP4_TAAGAAAACGCACTGCTTGC

labs

• [SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

References

• 10094672,12949160,12949159,9274030,28577341