opuE

proline transporter

locus: BSU_06660

pI: 9.81

mw: 53.12 kDa

protein length: 492 aa

gene length: 1479 bp

function: compatible solute transport

product: proline transporter

essential: no

synonyms: yerK

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.1|Solute:sodium symporter family]
• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.7|Sodium-solute symporter]
• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.9|Uptake of compatible solutes]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 726,840 728,318

Phenotypes of a mutant

• strong growth disadvantage in high-salinity minimal media lacking proline [Pubmed|22685134]

The protein

Protein family

• [SW|sodium:solute symporter (SSF) (TC 2.A.21) family] (according to UniProt)

Paralogous protein(s)

• [protein|B3DF99B747D0A7472E3878920BF3CE7CE954B0BE|PutP]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|6A1F759DAC09D364602460E5467E2761E97CE45C|opuE]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|9701821], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: activation, [Pubmed|22900538], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • induced by glucose ([protein|search|CcpA]) [Pubmed|22900538]

  additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

Biological materials

Mutant

• available in [SW|Erhard Bremer]'s lab [Pubmed|24142252]
• BKE06660 ([gene|6A1F759DAC09D364602460E5467E2761E97CE45C|opuE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTTTACCCTCTCTTCA, downstream forward: _UP4_TAAGCAATAAGCCATCAAGC
• BKK06660 ([gene|6A1F759DAC09D364602460E5467E2761E97CE45C|opuE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTTTACCCTCTCTTCA, downstream forward: _UP4_TAAGCAATAAGCCATCAAGC

labs

• [SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]

References

Reviews

• 27935846

Original publications

• 12884008,9701821,11902719,11902719,22685134,22900538,22383849,24142252,22139509,26728461