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opuE
function
compatible solute transport
product
proline transporter
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.1|Solute:sodium symporter family][category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.7|Sodium-solute symporter][category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.9|Uptake of compatible solutes][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]Gene
Coordinates
726,840 728,318
Phenotypes of a mutant
strong growth disadvantage in high-salinity minimal media lacking proline [Pubmed|22685134]The protein
Protein family
[SW|sodium:solute symporter (SSF) (TC 2.A.21) family] (according to UniProt)Paralogous protein(s)
[protein|B3DF99B747D0A7472E3878920BF3CE7CE954B0BE|PutP] [SW|Localization]
cell membrane (according to UniProt)Expression and Regulation
Operons
genes
[gene|6A1F759DAC09D364602460E5467E2761E97CE45C|opuE]
description
[Pubmed|22383849]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|9701821], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: activation, [Pubmed|22900538], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]regulation
induced by glucose ([protein|search|CcpA]) [Pubmed|22900538]additional information
the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]view in new tabBiological materials
Mutant
available in [SW|Erhard Bremer]'s lab [Pubmed|24142252]BKE06660 ([gene|6A1F759DAC09D364602460E5467E2761E97CE45C|opuE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTTTACCCTCTCTTCA, downstream forward: _UP4_TAAGCAATAAGCCATCAAGCBKK06660 ([gene|6A1F759DAC09D364602460E5467E2761E97CE45C|opuE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTTTACCCTCTCTTCA, downstream forward: _UP4_TAAGCAATAAGCCATCAAGClabs
[SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]References
Reviews
Original publications
12884008,9701821,11902719,11902719,22685134,22900538,22383849,24142252,22139509,26728461