SubtiBank SubtiBank
tsaB
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tsaB

required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA
locus
BSU_05920
pI
5.76
mw
25.10 kDa
protein length
229 aa Sequence Blast
gene length
690 bp Sequence Blast
function
tRNA modification
product
tRNA modification enzyme
essential
yes
synonyms
ydiC

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    642,111 642,800

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP ([protein|6CB350C335603D5F4434679F9D740BFECA738599|TsaB]-[protein|30393F16CFE89E7BAD233A0BD27B77D679F7E409|TsaD]-[protein|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|TsaE]) [Pubmed|23072323,21183954]
  • Protein family

  • KAE1 / TsaD family (with [protein|30393F16CFE89E7BAD233A0BD27B77D679F7E409|TsaD], according to UniProt)
  • Structure

  • [PDB|2A6A] (from ''Thermotoga maritima'', 24% identity) [Pubmed|20944216]
  • [SW|Localization]

  • cytoplasm (according to UniProt)
  • Expression and Regulation

    Operons

    genes
    [gene|DE32F1DA1EA1164411E0605262381BD38A49656C|thiL]-[gene|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE]-[gene|6CB350C335603D5F4434679F9D740BFECA738599|tsaB]-[gene|C2283BDD806221A285A27F24F52F92F4D47F0C55|ydiD]-[gene|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD]
    description
    [Pubmed|22383849]
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    References

  • 19376873,17005971,21285948,23072323,22383849,20944216,29741707