SubtiBank SubtiBank
nagR

nagR

transcriptional regulator (GntR family)
locus
BSU35030
pI
6.77
mw
27.36 kDa
protein length
243 aa Sequence Blast
gene length
729 bp Sequence Blast
function
regulator of the nagA-nagB-nagR operon
product
transcriptional regulator (GntR family)
essential
no
synonyms
yvoA

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.17|Utilization of amino sugars]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.3|Utilization of amino sugars]
  • Gene

    Coordinates
    3,597,289 → 3,598,020

    The protein

    Catalyzed reaction/ biological activity

  • transcription repression of ''[gene|7D5A8084FDDA962CCF028FC3735E68C4F2E77084|nagP]'' and of the putative ''[gene|8F4D075C9B9EF995E361F9BA5E8B52F42059C422|nagA]-[gene|0C8D7EAC2656E989E45B5B7E42FAA6D258956B56|nagB]-[gene|6D36D6360FE1CBFF5F64B2A6D1406405D6C6F7D5|nagR]'' operon in the absence of N-acetylglycosamine [Pubmed|24673833,20047956,21602348], weak repression of ''[gene|F2DE9BFE16D7B0DAE92DB58F9B1EDFBB7557ED16|yflG]'' [Pubmed|16207374]
  • Protein family

  • [SW|GntR family] of transcription factors
  • Kinetic information

  • K(D) for N-acetylglucosamine-6-phosphate: 1 mM [Pubmed|20047956]
  • Effectors of protein activity

  • N-acetylglucosamine-6-phosphate and glucosamine-6-phosphate act as the molecular inducers [Pubmed|25564531,21602348,20047956]
  • Structure

  • [PDB|2WV0] [Pubmed|20047956]
  • [PDB|4U0V] (complex with glucosamine-6-phosphate) [Pubmed|25564531]
  • [PDB|4U0W] (complex with N-acetylglucosamine-6-phosphate) [Pubmed|25564531]
  • [PDB|4WWC] (complex with palindromic operator DNA) [Pubmed|25564531]
  • Expression and Regulation

    Operons

    genes
    [gene|8F4D075C9B9EF995E361F9BA5E8B52F42059C422|nagA]-[gene|0C8D7EAC2656E989E45B5B7E42FAA6D258956B56|nagB]-[gene|6D36D6360FE1CBFF5F64B2A6D1406405D6C6F7D5|nagR]
    description
    [Pubmed|21602348]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|23667565], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|6D36D6360FE1CBFF5F64B2A6D1406405D6C6F7D5|NagR]: repression, [Pubmed|24673833,21602348], in [regulon|6D36D6360FE1CBFF5F64B2A6D1406405D6C6F7D5|NagR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • regulation

  • induced in the presence of N-acetylglucosamine ([protein|search|NagR]) [Pubmed|24673833,21602348,14343123]
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    Biological materials

    Mutant

  • MGNA-A335 (yvoA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/335 NBRP B. subtilis, Japan]
  • References

    Reviews

  • 26159076
  • Original publications

  • 19342794,20047956,16207374,21602348,23667565,24673833,25564531