cell shape-determining protein, forms filaments, the polymers control/restrict the mobility of the cell wall elongation enzyme complex, required for CwlO activity
function
cell shape determination
product
MreB-like protein
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.2|Cell shape][category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins][SW|Categories] containing this gene/protein
[SW|cell shape], [SW|sporulation proteins], [SW|membrane proteins]This gene is a member of the following [SW|regulons]
[SW|SigE regulon], [SW|stringent response]Gene
Coordinates on the chromosome (coding sequence)
3,747,254 -> 3,748,255
Phenotypes of a mutant
non-viable in the presence of low Mg(2 )readily accumulate ''[protein|search|rsgI]'' suppressor mutants [Pubmed|19114499]The protein
Catalyzed reaction/ biological activity
forms helical filaments in a heterologous system [Pubmed|21091501]required for [protein|search|CwlO] activity [Pubmed|23869552]Protein family
ftsA/mreB family (according to Swiss-Prot)Paralogous protein(s)
[protein|search|MreB], [SW|MreBH]Structure
[PDB|1JCF] ([SW|MreB] from ''Thermotoga maritima'', 52% identity) [Pubmed|11544518][SW|Localization]
during logarithmic growth, [protein|search|Mbl] forms discrete patches that move processively along peripheral tracks perpendicular to the cell axis [Pubmed|21636744]forms transverse bands as cells enter the stationary phase [Pubmed|21636744]close to the inner surface of the cytoplasmic membrane [Pubmed|16950129]reports on helical structures formed by Mbl [Pubmed|16950129,20566861] seem to be misinterpretation of data [Pubmed|21636744][SW|Interactions]
part of the [SW|cell wall biosynthetic complex] [Pubmed|21636744,21636745][protein|search|MreB]-[protein|search|Mbl] [Pubmed|21636744][protein|search|Mbl]-[SW|MreBH] [Pubmed|17064365][protein|search|RodZ]-[protein|search|Mbl] [Pubmed|23879732]Expression and Regulation
Operon
''[protein|search|usd]-[SW|spoIIID]-[protein|search|mbl]'' [Pubmed|22383849]''[protein|search|mbl]'' [Pubmed|22383849][SW|Sigma factor]
promoter p1 (upstream of ''[SW|usd]''): [protein|search|SigE] [Pubmed|9023218]promoter p2 (upstream of ''[SW|mbl]''): [protein|search|SigA] [Pubmed|26091431]Regulation
expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|9023218][protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]expression is strongly repressed in the presence of aminoguanidine (inhibitor of nitric oxide synthase) [Pubmed|22048160]Additional information
An [ncRNA|search|antisense RNA] is predicted for '[protein|search|mbl]' [PubMed|20525796]Biological materials
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW| Görke] labAntibody
available in the [SW|Jeff Errington] and [SW|Peter Graumann] labsLabs working on this gene/protein
[SW|Peter Graumann], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]References
Localization
17064365,20566861,21636744,21636745,22069484 Other original publications
19643765,16832063,11290328,12809607,16101995,12530960,7836311,16950129,9023218,19659933,19114499,21091501,20525796,22048160,22343529,23879732,23869552,26091431,22383849,27215790 
