SubtiBank SubtiBank
mbl [Mon May 30 2016 13:07:07 GMT+0200 (CEST)]
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mbl [Mon May 30 2016 13:07:07 GMT+0200 (CEST)]

cell shape-determining protein, forms filaments, the polymers control/restrict the mobility of the cell wall elongation enzyme complex, required for CwlO activity
35.00 kDa
cell shape determination
MreB-like protein

Genomic Context



  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.2|Cell shape]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW|Sporulation proteins/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [SW|Categories] containing this gene/protein

  • [SW|cell shape], [SW|sporulation proteins], [SW|membrane proteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|SigE regulon], [SW|stringent response]
  • Gene

    Coordinates on the chromosome (coding sequence)
    3,747,254 -> 3,748,255

    Phenotypes of a mutant

  • non-viable in the presence of low Mg(2 )
  • readily accumulate ''[protein|search|rsgI]'' suppressor mutants [Pubmed|19114499]
  • The protein

    Catalyzed reaction/ biological activity

  • forms helical filaments in a heterologous system [Pubmed|21091501]
  • required for [protein|search|CwlO] activity [Pubmed|23869552]
  • Protein family

  • ftsA/mreB family (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|search|MreB], [SW|MreBH]
  • Structure

  • [PDB|1JCF] ([SW|MreB] from ''Thermotoga maritima'', 52% identity) [Pubmed|11544518]
  • [SW|Localization]

  • during logarithmic growth, [protein|search|Mbl] forms discrete patches that move processively along peripheral tracks perpendicular to the cell axis [Pubmed|21636744]
  • forms transverse bands as cells enter the stationary phase [Pubmed|21636744]
  • close to the inner surface of the cytoplasmic membrane [Pubmed|16950129]
  • reports on helical structures formed by Mbl [Pubmed|16950129,20566861] seem to be misinterpretation of data [Pubmed|21636744]
  • [SW|Interactions]

  • part of the [SW|cell wall biosynthetic complex] [Pubmed|21636744,21636745]
  • [protein|search|MreB]-[protein|search|Mbl] [Pubmed|21636744]
  • [protein|search|Mbl]-[SW|MreBH] [Pubmed|17064365]
  • [protein|search|RodZ]-[protein|search|Mbl] [Pubmed|23879732]
  • Expression and Regulation


  • ''[protein|search|usd]-[SW|spoIIID]-[protein|search|mbl]'' [Pubmed|22383849]
  • ''[protein|search|mbl]'' [Pubmed|22383849]
  • [SW|Sigma factor]

  • promoter p1 (upstream of ''[SW|usd]''): [protein|search|SigE] [Pubmed|9023218]
  • promoter p2 (upstream of ''[SW|mbl]''): [protein|search|SigA] [Pubmed|26091431]
  • Regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|9023218]
  • [protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
  • expression is strongly repressed in the presence of aminoguanidine (inhibitor of nitric oxide synthase) [Pubmed|22048160]
  • Additional information

  • An [ncRNA|search|antisense RNA] is predicted for '[protein|search|mbl]' [PubMed|20525796]
  • Biological materials

    two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW| Görke] lab
  • Antibody

  • available in the [SW|Jeff Errington] and [SW|Peter Graumann] labs
  • Labs working on this gene/protein

  • [SW|Peter Graumann], Freiburg University, Germany [ homepage]
  • References


  • 17064365,20566861,21636744,21636745,22069484
  • Other original publications

  • 19643765,16832063,11290328,12809607,16101995,12530960,7836311,16950129,9023218,19659933,19114499,21091501,20525796,22048160,22343529,23879732,23869552,26091431,22383849,27215790