SubtiBank SubtiBank
mbl [2017-06-09 08:48:33]
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mbl [2017-06-09 08:48:33]

cell shape-determining protein, forms filaments, the polymers control/restrict the mobility of the cell wall elongation enzyme complex, required for CwlO activity
locus
BSU36410
pI
5.67
mw
35.70 kDa
protein length
333 aa Sequence Blast
gene length
999 bp Sequence Blast
function
cell shape determination
product
MreB-like protein
essential
yes
synonyms

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.2|Cell shape]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • Gene

    Coordinates
    3,747,254 → 3,748,255

    Phenotypes of a mutant

  • essential [Pubmed|28189581]
  • non-viable in the presence of low Mg(2 )
  • readily accumulate ''[gene|D6C2B8ABBBD1F9F2D36C34098D5B811A15C60D3B|rsgI]'' suppressor mutants [Pubmed|19114499]
  • The protein

    Catalyzed reaction/ biological activity

  • forms helical filaments in a heterologous system [Pubmed|21091501]
  • required for [protein|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|CwlO] activity [Pubmed|23869552]
  • Protein family

  • ftsA/mreB family (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB], [protein|40829C3632E9DCEB9E1C14A674B4B217F504CFE0|MreBH]
  • Structure

  • [PDB|1JCF] ([protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB] from ''Thermotoga maritima'', 52% identity) [Pubmed|11544518]
  • [SW|Localization]

  • during logarithmic growth, [protein|6ED386D49F973C1F6B1076974F54275DD583C9D3|Mbl] forms discrete patches that move processively along peripheral tracks perpendicular to the cell axis [Pubmed|21636744]
  • forms transverse bands as cells enter the stationary phase [Pubmed|21636744]
  • close to the inner surface of the cytoplasmic membrane [Pubmed|16950129]
  • reports on helical structures formed by Mbl [Pubmed|16950129,20566861] seem to be misinterpretation of data [Pubmed|21636744]
  • Expression and Regulation

    Operons

    genes
    [gene|6ED386D49F973C1F6B1076974F54275DD583C9D3|mbl]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, promoter p1, upstream of [protein|487990143C84D245743C0014E648699627204FF0|Usd] [Pubmed|9023218], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, promoter p2, upstream of [protein|6ED386D49F973C1F6B1076974F54275DD583C9D3|Mbl] [Pubmed|26091431], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]
  • regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|9023218]
  • additional information

  • An [ncRNA|search|antisense RNA] is predicted for '[protein|search|mbl]' [PubMed|20525796]
  • view in new tab

    genes
    [gene|487990143C84D245743C0014E648699627204FF0|usd]-[gene|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]-[gene|6ED386D49F973C1F6B1076974F54275DD583C9D3|mbl]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|9023218], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
  • regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|9023218]
  • additional information

  • An [ncRNA|search|antisense RNA] is predicted for '[protein|search|mbl]' [PubMed|20525796]
  • view in new tab

    Biological materials

    two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW| Görke] lab
  • Antibody

  • available in the [SW|Jeff Errington] and [SW|Peter Graumann] labs
  • Labs working on this gene/protein

  • [SW|Peter Graumann], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]
  • References

    Localization

  • 17064365,20566861,21636744,21636745,22069484
  • Other original publications

  • 19643765,16832063,11290328,12809607,16101995,12530960,7836311,16950129,9023218,19659933,19114499,21091501,20525796,22048160,22343529,23879732,23869552,26091431,22383849,27215790,28189581