SubtiBank SubtiBank
yclJ
Save the date! 20th International Conference on Bacilli and Gram-Positive Bacteria. Washington, D.C. July 23-26, 2019.

yclJ

two-component response regulator ([SW|OmpR family]), active under anaerobic conditions
locus
BSU_03750
pI
4.58
mw
26.36 kDa
protein length
227 aa Sequence Blast
gene length
684 bp Sequence Blast
function
unknown
product
two-component response regulator ([SW|OmpR family])
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.1|Two-component system response regulators]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.2|Phosphorylation on an Asp residue]
  • Gene

    Coordinates
    426,577 427,260

    The protein

    Protein family

  • [SW|OmpR family] of two-component response regulators
  • Paralogous protein(s)

  • [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR], [protein|6A37531896205A9B894C81AB0563C216C0B52CD7|YkoG], [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]
  • Modification

  • phosphorylated by [protein|52BB660B0CAB36C74F1D47963235846B45AF78AB|YclK] on an Asp residue
  • Effectors of protein activity

  • phosphorylation likely affects DNA-binding activity
  • Structure

  • [PDB|2OQR] (RegX3 from Mycobacterium tuberculosis, 42% identity) [pubmed|17942407]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|706983E6942E883D3A9D45693E7B4015AEABE60B|yclJ]-[gene|52BB660B0CAB36C74F1D47963235846B45AF78AB|yclK]
    description
    [Pubmed|15375128]

    regulatory mechanism

  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]: activation, [Pubmed|15375128], in [regulon|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD regulon]
  • regulation

  • expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|15375128]
  • view in new tab

    Biological materials

    Mutant

  • MGNA-C007 (yclJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2005 NBRP B. subtilis, Japan]
  • BKE03750 ([gene|706983E6942E883D3A9D45693E7B4015AEABE60B|yclJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATATCCTCCGGTTGTT, downstream forward: _UP4_ACTGTGTGGGGAGTAGGGTA
  • BKK03750 ([gene|706983E6942E883D3A9D45693E7B4015AEABE60B|yclJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATATCCTCCGGTTGTT, downstream forward: _UP4_ACTGTGTGGGGAGTAGGGTA
  • References

  • 10094672,20512483,15375128,23504016,17942407