citH

sporulation-specific secondary transporter of divalent metal ions/citrate complexes

locus: BSU_39060

pI: 9.28

mw: 45.37 kDa

protein length: 426 aa

gene length: 1281 bp

function: citrate uptake

product: secondary transporter of divalent metal ions/citrate complexes

essential: no

synonyms: yxiQ

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.4|Transporter for organic acids]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 4,010,404 4,011,684

The protein

Protein family

• [SW|CitM transporter family (TC 2.A.11)] (according to UniProt)

Paralogous protein(s)

• [protein|C0DED68802EF78D2BC16507234AAC3B60D236E68|CitM]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|71A73C825981E74842FC28D1B05CF85E9F3F5A56|citH]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|22383849], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

  regulation

  • expressed during [SW|sporulation] [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B723 (yxiQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1722 NBRP B. subtilis, Japan]
• BKE39060 ([gene|71A73C825981E74842FC28D1B05CF85E9F3F5A56|citH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATATGACCTCCCCTAT, downstream forward: _UP4_TAAATGTATGAGCTTGATCG
• BKK39060 ([gene|71A73C825981E74842FC28D1B05CF85E9F3F5A56|citH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATATGACCTCCCCTAT, downstream forward: _UP4_TAAATGTATGAGCTTGATCG

References

• 8892821,22383849,11053381